山东农业科学Issue(10)：30-34,5.
灌浆期高温对小麦旗叶中 SOD 和 GR 活性及相关基因表达量的影响
Effects of High Temperature at Filling Stage on Activities and Related Gene Expression of SOD and GR in Wheat Flag Leaves
摘要
Abstract
The effects of high temperature (HT) stress from the 10th day to the 20th day after anthesis on the activities and related gene expression of superoxide dismutase (SOD) and glutathione reductase (GR) were investigated with wheat cultivars Shannong 23 and Jimai 20 as materials .The results showed that SOD activity of Shannong 23 under HT stress was significantly higher than that of control , while that of Jimai 20 un-der HT stress initially increased and then declined compared with control .In Shannong 23 , the changes of the transcript levels of Fe-SOD and Mn-SOD were consistent with the change of SOD activity , but that of Cu/Zn-SOD was inconsistent .The expression changes of three SOD genes were basically consistent with that of SOD activity in Jimai 20 .The GR activity in two wheat cultivars under HT stress both initially increased and then declined .In Shannong 23 , the change of transcript level of GR was basically consistent with that of GR activity, but in Jimai 20,the change of GR was earlier than that of GR activity .Overall, Shannong 23 had higher antioxidant capacity to resist the HT stress than Jimai 20 .Fe-SOD and Mn-SOD played predominant roles in SOD activity.In addition, the related genes of antioxidant enzymes were more susceptible than enzyme activity in response to HT stress at filling stage .关键词
小麦/高温/超氧化物歧化酶/谷胱甘肽还原酶/基因表达Key words
Wheat ( Triticum aestivum L.)/High temperature/Superoxide dismutase/Glutathione re-ductase/Gene expression分类
农业科技引用本文复制引用
王春微,孙爱清,张杰道,尹燕枰..灌浆期高温对小麦旗叶中 SOD 和 GR 活性及相关基因表达量的影响[J].山东农业科学,2014,(10):30-34,5.基金项目
公益性行业（农业）科研专项经费（201203029）资助。 （）
