山东农业科学Issue(1):10-13,4.DOI:10.14083/j.issn.1001-4942.2015.01.003
水稻抽穗期基因Hd7m的定位及遗传分析
Mapping and Genetic Analysis of Heading Date Gene Hd7 m in Rice
摘要
Abstract
Using Hongqi 16 as the receptor and Minghui 63 as the donor, a BC4 F1 segregation population was developed through cross between the two parents and backcross with Hongqi 16.The early heading gene pool, late heading gene pool, and the parents were analyzed with 643 pairs of SSR markers, and 4 pairs of polymorphic markers were obtained.BC4 F2 populations derived from inbreeding of BC4 F1 hybrid plants with obvious segregation phenotype were selected for field investigation and genotype detection.The results showed that the target gene, named Hd7m, was linked to SSR marker PSM391, which located at the end of chromo-some 7.Based on the targeted interval, 10 pairs of new SSR markers were synthesized.Among which, the polymorphic marker RM22156 was apart from Hd7m of 4.1 cM.The results laid foundations for fine mapping, gene cloning and molecular marker assisted breeding of Hd7m.关键词
水稻/抽穗期/基因定位/SSR标记/遗传分析Key words
Rice/Heading date/Gene mapping/SSR marker/Genetic analysis分类
农业科技引用本文复制引用
曾莲,张华,柳絮,宣宁,李军,李广贤,杨永义,姚方印,刘开启..水稻抽穗期基因Hd7m的定位及遗传分析[J].山东农业科学,2015,(1):10-13,4.基金项目
国家自然科学基金项目(31171529)、山东省农业生物资源创新利用课题、山东省农业科学院科技创新重点项目(2014CXZ10-2)和山东省现代农业产业技术体系(水稻)项目资助。 ()