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水稻抽穗期基因Hd7m的定位及遗传分析

曾莲 张华 柳絮 宣宁 李军 李广贤 杨永义 姚方印 刘开启

山东农业科学Issue(1):10-13,4.
山东农业科学Issue(1):10-13,4.DOI:10.14083/j.issn.1001-4942.2015.01.003

水稻抽穗期基因Hd7m的定位及遗传分析

Mapping and Genetic Analysis of Heading Date Gene Hd7 m in Rice

曾莲 1张华 2柳絮 2宣宁 2李军 2李广贤 2杨永义 2姚方印 2刘开启1

作者信息

  • 1. 仲恺农业工程学院,广东 广州 510225
  • 2. 山东省农业科学院生物技术研究中心,山东 济南 250100
  • 折叠

摘要

Abstract

Using Hongqi 16 as the receptor and Minghui 63 as the donor, a BC4 F1 segregation population was developed through cross between the two parents and backcross with Hongqi 16.The early heading gene pool, late heading gene pool, and the parents were analyzed with 643 pairs of SSR markers, and 4 pairs of polymorphic markers were obtained.BC4 F2 populations derived from inbreeding of BC4 F1 hybrid plants with obvious segregation phenotype were selected for field investigation and genotype detection.The results showed that the target gene, named Hd7m, was linked to SSR marker PSM391, which located at the end of chromo-some 7.Based on the targeted interval, 10 pairs of new SSR markers were synthesized.Among which, the polymorphic marker RM22156 was apart from Hd7m of 4.1 cM.The results laid foundations for fine mapping, gene cloning and molecular marker assisted breeding of Hd7m.

关键词

水稻/抽穗期/基因定位/SSR标记/遗传分析

Key words

Rice/Heading date/Gene mapping/SSR marker/Genetic analysis

分类

农业科技

引用本文复制引用

曾莲,张华,柳絮,宣宁,李军,李广贤,杨永义,姚方印,刘开启..水稻抽穗期基因Hd7m的定位及遗传分析[J].山东农业科学,2015,(1):10-13,4.

基金项目

国家自然科学基金项目(31171529)、山东省农业生物资源创新利用课题、山东省农业科学院科技创新重点项目(2014CXZ10-2)和山东省现代农业产业技术体系(水稻)项目资助。 ()

山东农业科学

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1001-4942

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