山东农业科学Issue(2)：16-20,5.DOI:10.14083/j.issn.1001-4942.2015.02.004
芍药芽总 RNA 提取方法的研究
Study on Extraction Method of Total RNA from Peony Buds
摘要
Abstract
The total RNA was extracted from dormancy and dormancy -released peony buds by modified CTAB method , Trizol method and EASYspin Plus RNA rapid isolation kit respectively .The quality of the ex-tracted RNA was tested by protein nucleic acid analyzer , agarose gel electrophoresis and RT -PCR.The re-sults showed that the total RNA could not be extracted by Trizol method , and that extracted by EASYspin Plus RNA rapid isolation kit was too lower to meet the requirement of further experiment .The total RNA extracted by modified CTAB method was more complete with clearer bands and better quality , and the brightness of 28 S rRNA was about 2 times of that of 18S rRNA with no degradation .Using the total RNA extracted by modified CTAB method as template to amplify the internal reference gene Actin, the obtained bands were clear and with the same size to the target fragment , which further proved that the total RNA extracted by modified CTAB method could meet the requirement of the later experiment .关键词
芍药芽/总RNA/提取/RT-PCRKey words
Peony bud/Total RNA/Extract/RT-PCR分类
农业科技引用本文复制引用
胡小蓉,马燕,臧德奎,宋淑香,郭先锋..芍药芽总 RNA 提取方法的研究[J].山东农业科学,2015,(2):16-20,5.基金项目
山东省农业良种工程重大课题［鲁农良字（2010）6号］ （）
