山东农业科学Issue(2)：54-57,4.DOI:10.14083/j.issn.1001-4942.2015.02.014
铜绿假单胞菌 S8脂肪酶酶学性质及固定化研究
Study on Immobilization and Enzymatic Properties of Lipase from Pseudomonas aeruginosa S8
摘要
Abstract
The lipase produced by Pseudomonas aeruginosa S8 through flask fermentation was studied in enzymatic properties and immobilization .The results showed that the optimal reaction temperature and opti-mum pH value were 35℃and 7.0 respectively.The enzyme activity was stable at pH 6.0~8.0.The lipase was immoblilized on the carrier diatomite with absorption activity .The optimal immobilizing conditions were the mass ratio of carrier to enzymae as 10, immobilizing for 2.5 hours at 30℃and pH value as 7.5.关键词
铜绿假单胞菌/脂肪酶/酶学性质/固定化Key words
Pseudomonas aeruginosa/Lipase/Enzymatic properties/Immobilization分类
生物科学引用本文复制引用
曲威,刘惠军,薛元霞..铜绿假单胞菌 S8脂肪酶酶学性质及固定化研究[J].山东农业科学,2015,(2):54-57,4.基金项目
中国农业大学烟台研究院科研基金 （）
