山东农业科学Issue(3)：9-12,4.DOI:10.14083/j.issn.1001-4942.2015.03.003
桑青枯雷尔氏菌 gsp G和gsp K基因的获取与生物信息学分析
Acquisition and Bioinformatics Analysis of gsp G and gsp K Genes of Ralstonia solanacearum from Mulberry
摘要
Abstract
gsp G and gsp K genes are involved in the formation of periplasmic complex of Ralstonia so-lanacearum type Ⅱ secretion system .The sequences of gsp G and gsp K genes of Ralstonia solanacearum strain MR111 with 438 bp and 843 bp in length respectively were obtained from mulberry by PCR amplication and DNA sequencing technology .By online BLAST analysis , the similarity of the 2 genes with the homologous genes from other R.solanacearum species were both over 90%.There were typical functional structure of T2SG and T2SK, and secretory signal peptide composed mainly by leucine and alanine in the N -end in the 2 genes, respectively.A typeⅣsecretion system “Ⅳ_pilin_GFxxxE” structure was found in gsp G, and a re-peat sequence “GIQSTE” was found in gsp K.This repeat sequence was highly conserved in R.so-lanacearum.关键词
桑树/青枯雷尔氏菌/Ⅱ型分泌系统/gsp基因Key words
Mulberry/Ralstonia solanacearum/Type Ⅱsecretion system/gsp gene分类
农业科技引用本文复制引用
禚苏,孔卫青..桑青枯雷尔氏菌 gsp G和gsp K基因的获取与生物信息学分析[J].山东农业科学,2015,(3):9-12,4.基金项目
安康学院重点学科研究项目 （）
