山东农业科学Issue(5)：6-9,22,5.DOI:10.14083/j.issn.1001-4942.2015.05.002
基于 SSR 标记构建白蜡种质资源分子身份证
Establishment of Molecular ID for Fraxinus Germplasms Based on SSR Markers
摘要
Abstract
Using 46 Fraxinus materials from the Shouguang Experimental Station of Shandong Academy of Forestry , the establishment of molecular ID for Fraxinus germplasms was studied .These Fraxinus materials were distinguished by SSR markers .Eleven pairs of primers screened from 150 pairs were used to amplify the selected Fraxinus materials.The results showed that 3~5 genotypes could be detected by each primer .The average number of bands and polymorphic bands were 2.52 and 2.09 amplified by every pair of primers.The average polymorphic loci percentage was 82 .93%.After assignment of 11 pairs of primers and their alleles , the molecular IDs were established for the tested Fraxinus germplasms, which could well distinguish the varie-ties of Fraxinus.关键词
白蜡/SSR/分子身份证Key words
Fraxinus velutina Torr/SSR/Molecular ID分类
农业科技引用本文复制引用
胡春龙,张晨,刘翠兰,李丽,燕丽萍,吴德军,夏阳,邢世岩,王开芳..基于 SSR 标记构建白蜡种质资源分子身份证[J].山东农业科学,2015,(5):6-9,22,5.基金项目
国家科技支撑计划项目“白蜡种质资源发掘与创新利用”（2013BAD01B06－6）；山东省农业良种工程项目“农业生物资源创新利用研究”；山东省科技发展计划项目 （）
