山东农业科学Issue(6)：1-4,4.DOI:10.14083/j.issn.1001-4942.2015.06.001
洋葱细胞质雄性不育基因分子标记的开发
Development of a Molecular Marker Identifying Cytoplasmic Male Sterility Gene in Onion(Allium cepa L.)
摘要
Abstract
The specific primers of orfA501 in chives and cytoplasmic male sterile line of onion were used to PCR amplification.The amplified fragment was extracted and sequenced.The primers were designed ac-cording to the obtained sequence for genome walking,and partial flanking sequence was obtained.Then a SCAR marker was developed and named as OC2175.With this marker,a special fragment of 2 175 bp could be obtained from the male sterile lines and a 1 053 bp fragment was amplified from both (s)-cytoplasm and (N)-cytoplasm.Seventeen pairs of male sterile lines and their corresponding maintainer lines with different genetic backgrounds were used to verify this SCAR marker.The results indicated that the SCAR marker was perfectly suitable for distinguishing the cytoplasm types S or N in onion.关键词
洋葱/细胞质雄性不育/分子标记Key words
Onion/Cytoplasmic male sterility/Molecular marker分类
农业科技引用本文复制引用
李永博,刘冰江,霍雨猛,缪军,杨建平,吴雄,杨妍妍..洋葱细胞质雄性不育基因分子标记的开发[J].山东农业科学,2015,(6):1-4,4.基金项目
国家自然科学基金项目 （）
