山东农业科学Issue(6):97-100,4.DOI:10.14083/j.issn.1001-4942.2015.06.023
桃小食心虫滞育过程中5种代谢酶活力变化
Activity Changes of Five Metabolic Enzymes in Carposina sasakii Matsumura during Diapause
摘要
Abstract
To clarify the physiological adaptation mechanisms of overwintering,the activities of 5 meta-bolic enzymes,including trehalase,sorbitol dehydrogenase,hexokinase,pyruvate kinase and alkaline phos-phatase,of diapause larvae of Carposina sasakii Matsumura during different diapause time were measured in laboratory by kit.The results showed that the activities of trehalase,hexokinase,pyruvate kinase,alkaline phosphatase had significant differences between different diapause time,while those of sorbitol dehydrogenase had no significant difference.The trehalase activity was the highest in early diapause,then gradually de-creased with the prolongation of diapauses duration.The hexokinase activity was lower in early diapause,then increased rapidly and decreased rapidly in later diapause.The pyruvate kinase activity reached the highest lev-el in mid -diapause.The highest activity of alkaline phosphatase appeared in early diapause.These indicated that Carposina sasakii Matsumura improved their cold tolerance by regulating metabolic enzymes during dia-pause to ensure overwintering smoothly.关键词
桃小食心虫/越冬/滞育/代谢酶Key words
Carposina sasakii/Overwintering/Diapause/Metabolic enzyme分类
农业科技引用本文复制引用
张顺益,秦华伟,任凤山,刘廷利,陈珍珍,门兴元,于毅,许永玉,李丽莉..桃小食心虫滞育过程中5种代谢酶活力变化[J].山东农业科学,2015,(6):97-100,4.基金项目
国家公益性行业(农业)科研专项(201103024);山东省现代产业技术水果体系创新团队项目 ()