山东农业科学Issue(6)：118-124,7.DOI:10.14083/j.issn.1001-4942.2015.06.029
牡丹皮中多糖提取工艺的响应曲面法优化研究
Optimization of Extraction Technology of Polysaccharides from Moutan Cortex via Response Surface Methodology
摘要
Abstract
To efficiently extract polysaccharides from moutan cortex,the response surface methodology was used to optimize the extraction technology.The Box -Behnken design method was used to conduct the 3 -factors and 3 -levels experiment on the basis of single -factor experiment for the responsive surface analysis. The extraction temperature,extraction time and ratio of water to material were the response factors.The yield of polysaccharides was the response value.The results showed that the yield of polysaccharides were signifi-cantly affected by extraction temperature and time.The effects of the 3 factors on polysaccharide yield were in order of extraction temperature,extraction time and ratio of water to material.The optimum extraction condi-tions were the extraction temperature as 84℃,extraction time of 1.1 hours and ratio of water to material as 31∶1.The predicted value of polysaccharide yield was 3.732% and the verified value was 3.727%.The veri-fied value was basically coincident with the predicted one with the relative error as 0.134%.This study could provide theoretical references for deep processing of moutan cortex and lay the foundation for studying on the structure -function relationship of polysaccharides from moutan cortex.关键词
牡丹皮/多糖/响应面分析/优化Key words
Moutan cortex/Polysaccharide/Responsive surface analysis/Optimization分类
农业科技引用本文复制引用
张利霞,常青山,侯小改,侯典云,薛俊鹏,徐凯锋,李亚鸽..牡丹皮中多糖提取工艺的响应曲面法优化研究[J].山东农业科学,2015,(6):118-124,7.基金项目
河南省高等学校重点科研项目（15A180037）；河南科技大学博士科研启动基金项目（09001697，09001690）；河南科技大学青年基金项目（4024－13350065，4026－13350041）；河南科技大学大学生科研训练计划（SRTP）项目 （）
