山东医药Issue(9)：1-3,3.DOI:10.3969/j.issn.1002-266X.2014.09.001
温石棉及其代用纤维对 V79细胞 p16和 p53表达的影响
Effects of chrysotile and its substitute on p16 and p53 expression of V79 hamster cells
摘要
Abstract
Objective To investigated the effects and possible carcinogenic mechanism of chrysotile and its substitute on p16 and p53 expression of V79 hamster cells.Methods Chinese hamster lung cells (V79 cells) were exposed to the suspension of chrysotile and its substitute [ ( Xinkang Sichuan ( A ) , chrysotile from Southern Shanxi ( B ) , glass fiber (C), ceramic fiber (D), wollastonite (E) and rockwool (F) groups and control group (G)] with different powder con-tent (1-10 mg/L).The p16 and p53 expression in V79 cells was tested by immunohistochemistry method , then p16 and p53 statistics were compared .Results Compared with the control group , the antioncogene expression of p 16 and p53 was down-regulated in V79 cells of the six experiment groups (P<0.05).With the increase of exposure concentrations , the expression of p16 and p53 were decreased and OD value of p16 and p53 were decreased (all P<0.05).Conclusions Chrysotile and its substitute show different levels of carcinogenicity .The carcinogenic mechanism of chrysotile and its sub-stitute may be related to down-regulation of the tumor gene expression of p 16 and p53 .关键词
温石棉/代用品纤维/基因表达调控,肿瘤/基因,p16/基因,p53Key words
chrysotile/substitute/tumor gene/genes expression regulation , neoplastic/genes, p16/genes, p53分类
医药卫生引用本文复制引用
王洪州,邓丽娟,王利民,董发勤..温石棉及其代用纤维对 V79细胞 p16和 p53表达的影响[J].山东医药,2014,(9):1-3,3.基金项目
国家自然科学基金资助项目（49502025及40072020）；四川省科技厅科研基金项目（2010SZ0037）。 （）
