山东医药Issue(10)：14-16,20,4.DOI:10.3969/j.issn.1002-266X.2014.10.005
Notch1 siRNA 对小鼠恶性黑色素瘤细胞体内外增殖的抑制作用研究
Inhibitory effect of Notch1 siRNA on proliferation and tumor growth of malignant melanoma cells in vivo and in vitro
摘要
Abstract
Objective To observe the inhibit effeet of siRNA on Notch 1 in mouse malignant melanoma cells in vivo and vitro.Method We used siRNA targeting Notch1 gene to transfect melanoma cell line B16F1and induced RNA inter-ference.RT-PCR, Western blotting, MTT method and cell cloning assay in vitro were used to detect the expression of Notch1 gene and protein expression , cell proliferation and cell clone formation changes .Then we transfected or inoculated the control cells subcutaneously into syngeneic C 57BL/6 mice, and in the intratumoral process , the corresponding siRNA was injected .The tumor growth was recorded and the growth curve was drawn .After that , we executed the animal to get tumor and compared the tumor volume .Meanwhile, HE and immunohistochemical staining were used to observe the patho-logical changes of tumor tissue and the expression of Notch 1 protein.Results The cell proliferation , clone formation rate and tumor formation in mice were inhibited after Notch1 siRNA transfecting B16F1 cells (P<0.01), and immunohisto-chemistry showed that Notch1 protein positive cell percentage of the siNotch 1 transfected group was lower than that of con-trol group (all P<0.01).Conclusions Targeting Notch1 gene siRNA can inhibit the proliferation and growth of mouse melanoma cells by decreasing the expression of Notch 1, which can be used as a new method for the treatment of malignant melanoma.关键词
恶性黑色素瘤,小鼠/细胞增殖/靶向Notch1基因小干扰RNAKey words
malignant melanoma , mice/cell proliferation/targeting Notch1 gene small interfering RNA分类
医药卫生引用本文复制引用
齐艳霞,康世均,刘新会,罗荣城..Notch1 siRNA 对小鼠恶性黑色素瘤细胞体内外增殖的抑制作用研究[J].山东医药,2014,(10):14-16,20,4.基金项目
广东省自然科学基金重点项目（10251051501000008）。 （）
