山东医药Issue(22)：5-7,3.DOI:10.3969/j.issn.1002-266X.2014.22.002
ML-7灌胃对动脉粥样硬化兔肾组织中微量元素含量的影响
Influence of ML-7 intragastric administration on contents of trace elements in rabbits′kidney with atherosclerosis
摘要
Abstract
Objective To explore the influence of myosin light chain kinase ( MLCK) inhibitor ML-7 on the contents of Fe, Mn, Cu, Zn, Se, Pb and Cd in rabbits'kidney with atherosclerosis ( AS) .Methods Forty rabbits were randomly divided into 3 groups:ML-7 treatment group (n=17), high-fat diet group (n=13) and normal control group (n=10). Rats in the ML-7 treatment group were fed with high-fat food, from the 6th week on, 5 mg/(kg· d) ML-7 was given via intragastric administration for 12 weeks.High-fat feeding was given to rats in the high-fat diet group for 12 weeks.Rats in the normal control group were given basal feeding for 12 weeks.After feeding for 12 weeks, blood was collected from the carotid of the rabbits in each group after anesthesia .Then kidney tissue were taken immediately after the killing of the rab-bits.TC level in the carotid blood was detected , and the contents of Fe , Mn, Cu, Zn, Se, Pb and Cd in the kidney of each group was analyzed by using ICP-AES.Results TC levels in the ML-7 treatment group , high-fat diet group and nor-mal control group were (21.68 ±4.69), (28.33 ±1.95) and (1.03 ±0.70) mmol/L respectively.TC levels in the ML-7 treatment group and high-fat diet group were higher than that of the normal control group , while TC level in the ML-7 treatment group was lower than that of the high fat diet group (all P<0.05).Compared with the normal control group , the contents of Zn, Mn and Se decreased, while Fe content increased (all P<0.05).Compared with the high-fat diet group, the contents of Zn, Mn and Se increased, while Fe content decreased (all P<0.05).Conclusions ML-7 can decrease TC level in the serum of model rabbits , increase Zn, Mn and Se contents in rabbits'kidney with AS.Meanwhile, ML-7 plays a role of resistance to the AS by increasing the Zn , Mn and Se contents in the kidney .关键词
肌球蛋白轻链激酶/动脉粥样硬化/肾脏/微量元素Key words
myosin light chain kinase/atherosclerosis/kidney/trace elements分类
化学化工引用本文复制引用
吴允凯,王虎,韦文美,顾志红,汪渊..ML-7灌胃对动脉粥样硬化兔肾组织中微量元素含量的影响[J].山东医药,2014,(22):5-7,3.基金项目
国家自然科学基金资助项目（30971226）。 （）
