山东医药Issue(23)：60-63,4.DOI:10.3969/j.issn.1002-266X.2014.23.021
宫颈细胞 HPV E6／E7 mRNA 检测在宫颈病变诊断中的价值
Clinical value of branch-DNA technique for human papillomavirus E6/E7 mRNA detection in cervical lesions
摘要
Abstract
Objective To evaluate the clinical value of human papillomavirus ( HPV) E6/E7 mRNA detection in diagnosis of cervical lesions.Methods Totally 520 patients were examined with b-DNA technology to detect HR-HPV E6/E7mRNA and the cervical cytological examination with liquid-based cytology (TCT).Women with cytologi-cal abnormalities or HR-HPV E6/E7 mRNA positive results underwent colposcopy and cervical biopsy .We compared the sensitivity, specificity, positive predictive value and negative predictive value between the HPV E6/E7mRNA and TCT for the detection of high-grade cervical intraepithelial neoplasias ( CIN) and cervical cancer ( CC) .Results A-mong 520 patients, 158 cases were found HPV E6/E7mRNA positive and 222 cases got TCT abnormal.The positive rate of HPV E6/E7 mRNA with abnormal results in TCT abnormal group was higher than that of the normal group ( P<0.05) .There was difference in the positive rate of HPV E6/E7mRNA between the group who got chronic cervicitis or low-grade intraepithelial neoplasias (CINⅠ) and the group who got high-grade CIN and CC (P<0.05).For the detection of high-grade CIN and CC, the sensitivity of HPVE6/E7 mRNA and TCT was 74.19% and 81.53%, re-spectively; the specificity was 83.33% and 69.44%, respectively; the positive predictive value was 58.23% and 81.45%, respectively; and the negative predictive value was 71.43% and 92.28% respectively.The specificity and positive predictive value was statistically different between the two groups (all P<0.05).Conclusion In cervi-cal cancer screening, high-risk HPV E6/E7 mRNA detection in predicting high-grade CIN has high clinical value, es-pecially for assessing the risk of malignancy.关键词
b-DNA技术/人乳头状瘤病毒/E6基因/E7基因/宫颈高度上皮内瘤样病变Key words
b-DNA technology/human papillomavirus/E6 gene/E7 gene/high-grade cervical intraepithelial neo-plasias分类
医药卫生引用本文复制引用
严雪梅,洪颖..宫颈细胞 HPV E6／E7 mRNA 检测在宫颈病变诊断中的价值[J].山东医药,2014,(23):60-63,4.基金项目
南京市医学科技发展项目（YKK13189）。 （）
