山东医药Issue(24)：16-18,3.DOI:10.3969/j.issn.1002-266X.2014.24.006
STAT3、Cyclin D1在大鼠肝癌组织中的表达变化及意义
Expression changes and significance of STAT3 and cyclin D1 in hepatocellular carcinoma tissues of rats
摘要
Abstract
Objective To observe the expression changes of signal transducer and activator of transcription 3 (STAT3) and cyclin D1 in hepatocellular carcinoma tissues of rats and to investigate its significance .Methods Seventy-two rats were randomly divided into the model group and control group , 36 rats in each group .The model group received 0.1 mg/mL diethylnitrosamine (DEN) freely, and the control group was given the same volume of sterile distilled water for 20 weeks.The STAT3 and cyclin D1 mRNA was detected by using RT-PCR, Western blotting and immunohistochemistry were used to detect the STAT 3 and cyclin D1 protein expression .Results In control group , the mRNA expression of STAT3 and cyclin D1 were respectively 0.32 ±0.12 and 0.18 ±0.05, in the model group respectively 0.72 ±0.25 and 0.57 ±0.15, all P<0.05;in the control group, the protein expression levels of STAT3 and cyclin D1 were respectively 0.32 ±0.19 and 0.41 ±0.15, while in the model group , they were respectively 0.58 ±0.25 and 0.65 ±0.14, all P<0.05;immunohistochemical results showed that the IOD of STAT 3 and cyclin D1 in the model group was respectively 2 373.87 ±258.79 and 668.44 ±63.10, in control group, it was respectively 487.81 ±55.31 and 138.86 ±31.50, all P<0.05.Conclusion The expression of STAT3 and cyclin D1 in the hepatocellular carcinoma tissues of rats is up-regula-ted, and both of them can promote the occurrence and development of hepatocellular carcinoma .关键词
肝肿瘤/原发性肝细胞癌/二乙基亚硝胺/信号转导和转录活化因子3/细胞周期素D1/大鼠Key words
liver neoplasms/primary hepatocellular carcinoma/diethylnitrosamine/signal transducer and activator of transcription 3/cyclin D1/rats分类
医药卫生引用本文复制引用
买热艳木·艾尔肯,热沙来提·阿不都瓦衣特,王岩蛟,依马木·买买提明,日斯拉特·艾力木,斯坎德尔·白克力..STAT3、Cyclin D1在大鼠肝癌组织中的表达变化及意义[J].山东医药,2014,(24):16-18,3.基金项目
国家自然科学基金资助项目（81160545）。 （）
