山东医药Issue(25)：7-9,3.DOI:10.3969/j.issn.1002-266X.2014.25.004
NLRP3炎性体加重小鼠脑缺血再灌注损伤的机制探讨
Effect of NLRP3 inflammasome on cerebral ischemia reperfusion injury of mice and the mechanism
摘要
Abstract
Objective To investigate the effect of NLRP 3 inflammasome in the development of cerebral ischemia reperfusion injury in mice and the mechanism .Methods All of the C57 BL/6 mice were randomly divided into two groups , 20 in each group:the control group and glyburide group .Glyburide was used as inhibitor of NLRP 3 inflammasome and a mouse model of cerebral ischemia reperfusion was established by middle cerebral artery occlusion ( MCAO ) .Ten mice of each group were subjected to MCAO to make the ischemia reperfusion models , and others underwent sham opera-tion.Glyburide 500 mg/kg was given to the mice in the glyburide group by intraperitoneal injection 30 min before surgery. After MCAO, neurological scoring and TTC staining were conducted and the ischemic infarction area was calculated .The pathological change and neuronal apoptosis in brain tissues were observed with HE staining and TUNEL staining , respec-tively.The expression of NLRP3 and cytokines ( IL-1β, IL-18) was identified by Western blotting and ELISA , respective-ly.Results The degree of brain injury in IR mice of each group was more serious than that of the sham operation group , but the IR mouse brain damage of the control group was more serious than that of glyburide group .HE staining and TUNEL staining indicated significant injuries were found in the brain tissues of IR mice , the amount of neuronal apoptosis was in-creased, and the glyburide reduced the degree of pathological injuries caused by reperfusion .Compared with the control group, the increasing degree in expression of NLRP3 protein and cytokines (IL-1β, IL-18) was significantly decreased in the glyburide group (all P<0.05).Conclusion NLRP3 inflammasome promotes the development of cerebral ischemia reperfusion injuries by increasing cytokine release and enhancing the neuron apoptosis .关键词
缺血再灌注损伤/Nod样受体蛋白3炎性体/格列本脲/白细胞介素1β/白细胞介素18/小鼠Key words
cerebral ischemia reperfusion injury/NLRP3 inflammasome/glyburide/interleukin-1β/interleukin-18/mice分类
医药卫生引用本文复制引用
易敏,高荔,庞博,杨帆,庞琦..NLRP3炎性体加重小鼠脑缺血再灌注损伤的机制探讨[J].山东医药,2014,(25):7-9,3.基金项目
国家自然科学基金项目（81171062）；山东省国际科技合作计划（2011GHZ21801）；山东省自然科学基金项目（ZR2010HM112）。 （）
