山东医药Issue(29)：8-11,4.DOI:10.3969/j.issn.1002-266X.2014.29.004
塞来昔布联合 NK 细胞对裸鼠人肝癌皮下移植瘤生长的影响
Combined effects of celecoxib and NK Cells on growth of SMMC-7721 hepatocellar carcinoma xenografts in nude mice
摘要
Abstract
Objective To evaluate the inhibitory effect and its mechanism of celecoxib combined with NK cells on the growth of implanted human liver caner cell line SMMC-7721 in nude mice.Methods Forty nude mice were inoculated the hepatoma cell line SMMC-7721, and then were randomly divided into the control group , NK cells group, celecoxib group and combined intervention group with 10 rats in each group.in the NK cells group, the mice were intratumorally injected NK cell suspension 0.6 mL, every 7 d for one time, for 5 times;in the celecoxib group, from the third day after inocula-tion, the mice were treated with celecoxib 100 mg/kg orally, 1 time a day for 35 days;in the combined intervention group , mice were given celecoxib and NK cells , whose dosage and way were the same as that of the single group; in the control group, mice were given oral and intratumoral injection of normal saline .Thirty-five days later , the mice′s tumors were ex-cised for further analysis .The tumors were weighed and the tumor inhibitory rate was calculated .Apoptosis was measured by using a terminal deoxynucleotidyl transferase-mediated nick-end labeling ( TUNEL) assay.The protein levels of vascular endothelial growth factor (VEGF), Bax, Bcl-2, caspase-3, Ki-67 and NF-κB were detected by immunohistochemistry . Results The tumor volume , tumor mass and apoptosis index of the combined intervention group were significantly lower than those of the NK cells group and celecoxib group (all P<0.05).The tumor inhibition rate was 28.84% in the NK cells group and 50.42%in the celecoxib group which was significantly lower than that 87.59%in the combined interven-tion group.The expression of VEGF, Bcl-2, NF-κB and Ki-67 was significantly lower, but the expression of Bax and caspase-3 was significantly higher in the combined intervention group than that of the other single groups (all P<0.05).Conclusion Celecoxib combined with NK cells can induce the apoptosis of SMMC-7721 cells and inhibit the growth of xenografts, whose mechanism may be related to inhibiting the expression of VEGF , Bcl-2, NF-κB, Ki-67 and promoting the expression of Bax and caspase-3.关键词
肝肿瘤/塞来昔布/自然杀伤细胞Key words
liver neoplasms/celecoxib/natural killer cells分类
医药卫生引用本文复制引用
陈超,郭雪兴,刘军权,陈剑群..塞来昔布联合 NK 细胞对裸鼠人肝癌皮下移植瘤生长的影响[J].山东医药,2014,(29):8-11,4.基金项目
徐州医学院研究生科技创新基金（XYYC-1207）。 （）
