山东医药Issue(32)：20-22,112,4.DOI:10.3969/j.issn.1002-266X.2014.32.007
白藜芦醇对胃癌 SGC7901细胞自噬的影响及其机制探讨
Effect of resveratrol on the autophagy of SGC7901 cells and its mechanism
摘要
Abstract
Objective To study the influence of resveratrol ( RES) on the autophagy of SGC7901 cells and the role of classⅢPI3K.Methods SGC7901 cells were treated with different dose of RES (0, 10, 20, 50, 75, 100 μmol/L) for 24 h alone or pretreated with 3-MA ( or wortmannin, or chloroquine) for 1 h.Cell lysates were analyzed by Western blot for LC3-Ⅰ/LC3-Ⅱconversion;MDC staining was used to detect the morphologic changes of autophagy.Results The LC3-Ⅰ/LC3-Ⅱconversion and the number of autophagosomes increased in a dose dependent of RES and markedly increased in the presence of the protease inhibitors chloroquine.And the inhibitory function of class Ⅲ PI3K by its specific inhibitors ( wortmannin) had no impact on the level of autophagy induced by RES.Conclusion RES increased the autophagy of SGC7901 cells, and the autophagy was independent of classⅢPI3K.关键词
白藜芦醇/胃肿瘤/自噬/Ⅲ型PI3K/胃癌细胞Key words
resveratrol/stomach cancer/autophagy/class ⅢPI3K/gastric cancer cell lines分类
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才明洁,庞晓阳,朱丹,董莹玥..白藜芦醇对胃癌 SGC7901细胞自噬的影响及其机制探讨[J].山东医药,2014,(32):20-22,112,4.
