山东医药Issue(33)：13-16,4.DOI:10.3969/j.issn.1002-266X.2014.33.005
健脾解毒方逆转大肠癌细胞多药耐药的作用机制
Mechanism of multidrug resistance of colon cancer cell reversed by Jianpijiedu Herb
摘要
Abstract
Objective To investigate the mechanism of which Chinese traditional medicine , Jianpijiedu ( JBJD) herb protocol reverses multidrug resistance ( MDR) of colon cancer .Methods Sensitivity of Vincristine to colon cancer cells ( HCT-8/V) were detected by cell growth inhibition metods befor or after the treatment of Jianpijiedu Herb .Expression of multidrug gene 1 ( MDR-1) and phosphorylation of Akt in cancer cell were studied by either real -time PCR or Western blot.Results After HCT-8/V was co-cultured with serum containing 20% of JBJD for 24 h, 48 h and 72 h, inhibition rates on proliferation of HCT-8/V resulted by Vincristine were 5.9%, 11.0% and 15.0%, respectively, and all higher than that of the blank control group (all P<0.05).Inhibiting cell growth of Vincristine was positively correlated to the co-culture time and the serum concentration of JBJD .JBJD down-regulated the expression of MDR-1 protein and mRNA in HCT-8/V(P<0.05).Expression of MDR-1 was positively related with the decreased degree of Akt phosphorylation induced by JBJD, and negatively correlated with sensitivity of Vincristine .JBJD combined with PI3K inhibitor(LY294002) resulted in significant deduction of MDR-1 expression at mRNA and protein levels (P-gp) and Akt phosphorylation (p-Akt), and enhanced Vincristine sensitivity significantly than that by single use of JBJD or LY 294002 .Conclusion JBJD effectively turn down activity of PI 3K/Akt signaling pathways by deduction of cellular Akt phosphorylation , which down-regulate the expression of MDR1, increase the sensitivity of Vincristine .关键词
大肠肿瘤/健脾解毒方/多药耐药基因/PI3 K/Akt信号因子Key words
colon cancer/Jianpijiedu herb/multidrug resistance/PI3K/Akt signaling分类
医药卫生引用本文复制引用
李先茜,吴嘉熙,范忠泽,孙燕妮,高虹..健脾解毒方逆转大肠癌细胞多药耐药的作用机制[J].山东医药,2014,(33):13-16,4.基金项目
3项，上海市启明星项目1项。获上海市科技成果奖2项，上海医学科技奖三等奖1项，申请发明专利3项。近年发表论文30篇（其中在SCI上发表4篇）。 E-mailxeexee＠126．com （）
