山东医药Issue(34)：21-23,3.DOI:10.3969/j.issn.1002-266X.2014.34.007
胶质细胞源性神经营养因子对帕金森病大鼠多巴胺能神经元的修复作用
Restorative effect of glial cell line-derived neurotrophic factor by intracerebral infusion on nigrostriatal dopaminergic neurons in rat Parkinson model
摘要
Abstract
Objective To evaluate the restorative effect of glial cell line-derived neurotrophic factor ( GDNF) intrace-rebrallyinfusedonnigrostriataldopaminergicneuronsinratmodelwithParkinsoniandisease.Methods 70ratswereem-ployed for the modeling of Parkinsonian disease with right intracerebral( medial forebrain bundle,MFB; ventral tegmental area,VTA) infusion of 12μg 6-OHDA( 3μg /μL) ,and get 39 rat models.29 rats were received controlled intracerebral human recombined GDNF infusion (intraventricle:n=15, intraputamen:n=14) , while the other 10 rats were given ve-hicle as controls( intraventricle:n=5, intraputamenal: n=5).Immunohistochemistry and quantitative morphological a-nalysis was used to quantify the number and size of tyrosine hydroxylase positive( TH+) dopaminergic neurons and the TH+fiber density in nigrostriatal region.Results In controlled group ,the number and density of TH+dopaminergic neuron-swere in right sids increased than left.(P<0.05).Compared to controls,TH+fiber increased bilaterally in number, size and density in periventricular striatum of lesioned side.Conclusions Intracerebral GDNF infusion induces significant re-storative effect on nigrostriatal dopaminergic neurons.关键词
帕金森病/胶质细胞源性神经营养因子/多巴胺/神经元Key words
glial cell line-derived neurotrophic factor/parkinsonism disease/immunohistochemistry/quantitative morphology分类
医药卫生引用本文复制引用
许加军,杨洪安,王国栋..胶质细胞源性神经营养因子对帕金森病大鼠多巴胺能神经元的修复作用[J].山东医药,2014,(34):21-23,3.基金项目
山东省优秀中青年科学家奖励基金项目（2004BS03017）。 （）
