山东医药Issue(36)：4-7,4.DOI:10.3969/j.issn.1002-266X.2014.36.002
超高效液相色谱分析条件变化对汉黄芩苷转化因子测定的影响
Effects of UPLC analysis conditions on the conversion factor determination of wogonoside
摘要
Abstract
Objective To investigate the effects of UPLC analysis conditions on the conversion factor ( K) determina-tion of wogonoside .Methods A series of standard working solutions containing wogonoside and wogonin ( molar ratio 1∶1 ) were prepared and determined by UPLC to obtain peak area ratio of the two compounds , which was equal to the value of K.Then, analysis conditions were altered respectively and observed the variance of K value .Results When pH value of mobile phase A rose from 2.5 to 3.5, 4.5 and 6.5, K values were found to increase 10.7%-14.6%.When the pH value rose to 8.5, the retention time of wogonoside and wogonin moved forward significantly , which affected the accuracy of K value determination .With the variance of ionic strength of mobile phase A and pH value of sample medium , the K values determined were similar (deviation<4%).With the variance of detection wavelength from predetermined 280 nm to 254, 274, 342 nm, deviation of K value was in the range of 4.1%-10.7%.Conclusions The K value determination of wogonoside is susceptible to the variance of several UPLC analysis conditions such as pH value of mobile phase A , detection wavelength .To provide more precise quanification for glucuronides which are usually not available commercially , it is nec-essary to keep UPLC analysis conditions consistent during the process of determination .关键词
转化因子/UPLC分析条件/汉黄芩苷/汉黄芩素Key words
conversion factor/UPLC analysis conditions/wogonoside/wogonin分类
医药卫生引用本文复制引用
周琼,石剑,王桂平,钱芳,张彥焘,夏笔军..超高效液相色谱分析条件变化对汉黄芩苷转化因子测定的影响[J].山东医药,2014,(36):4-7,4.基金项目
国家自然科学基金资助项目（81102506／H3110）；广州医学院青年科学基金资助项目（2010A02）。 （）
