山东医药Issue(36)：18-21,4.DOI:10.3969/j.issn.1002-266X.2014.36.006
贵阳地区汉族人群 HPA-1～5基因多态性与2型糖尿病合并颈动脉粥样硬化的关系
Analysis of association between genetic polymorphism of human platelet antigens HPA-1-5 and type 2 diabetes combined with carotid atherosclerosis of Han people in Guiyang
摘要
Abstract
Objective To study the association between genetic polymorphism of human platelet antigens HPA -1-5 and type 2 diabetes combined with carotid atherosclerosis ( CA) of Han people in Guiyang .Methods 99 patients with type 2 diabetes (diabetes group) and 100 healthy controls (normal group) were enrolled in this case-control study.The di-abetes group was devided into two groups , which was CA( +) (combined with CA) and CA( -) (non-CA).Phenol-chloroform method was used to extract the genomic DNA and sequence-specific primers-polymerase chain reaction ( SSP-PCR) technique was applied for amplification of the target gene and genotyping .Results The allele frequency and geno-type of HPA-1, 2, 3, 5 showed significant differences among the normal group , CA(+) group and CA(-) group (all P<0.05).The allele frequency and genotype of HPA-1, 2, 3 showed significant differences between the normal group and the diabetes group (all P<0.05).The allele frequency and genotype of HPA-5 showed significant differences between CA (+) and CA(-) (P<0.05).Logistic regression analysis showed that age , duration of diabetes, hypertension, smok-ing, overweight, dyslipidemia, genetic polymorphism of HPA-5 were risk factors of CA in patients with type 2 diabetes. Conclusions There is a possible correlation between genetic polymorphism of human platelet antigens HPA -1, 2, 3 and occurrence of type 2 diabetes.Genetic polymorphism of HPA-5 may be the risk factor of type 2 diabetes with CA.关键词
人类血小板抗原/基因多态性/2型糖尿病/动脉粥样硬化Key words
human platelet antigens/genetic polymorphism/type 2 diabetes/atherosclerosis分类
医药卫生引用本文复制引用
郑婧,徐时方,张亚华,洪欢山,范良敏..贵阳地区汉族人群 HPA-1～5基因多态性与2型糖尿病合并颈动脉粥样硬化的关系[J].山东医药,2014,(36):18-21,4.基金项目
贵州省贵阳市科技局科研课题计划项目［2012（24）］。 （）
