山东医药Issue(38)：5-7,3.DOI:10.3969/j.issn.1002-266X.2014.38.002
国产地西他滨对小鼠淋巴瘤／白血病细胞EL4形态、增殖及CD80表达的影响
Effect of domestic decitabine influence on morphology, proliferation and CD80 expression in mouse lymphoma/leukemia cell line EL4 cells
摘要
Abstract
Objective To compare the influence of cytotoxicity and CD80 expression in EL4 cells, following in vitro treatment with decitabine ( DEC) manufactured internal.Methods EL4 cell line was treated with Dacogen ( DAC, manu-factured by Xian Janssen Pharmaceutical Ltd) or DEC (final concentration 0.25μmol/L) in vitro for 3 days.Morphology was analysised by method of Wright-Gimesa staining.CD80 expression was detected by method of flow cytometry.Cell pro-liferation was detected by method of CCK-8 kit.Results DAC and DEC treatment resulted in similar morphological chan-ges in EL4 cells.Moreover, effects of CD80 expression and inhibited proliferation induced by both DAC and DEC in EL4 cells were no significant differences (P>0.05).Conclusions There was no significant difference between DAC and DEC in the ability of change cellular morphology, inhibit proliferation and stimulate CD80 expression in mouse-lymphoma/leuke-mia cell EL4 cultured in vitro, which indicates the effects of the two drugs were similarity in both cytotoxic effect on tumor cells and stimulation of anti-tumor immune response when administered in vivo.关键词
地西他滨/淋巴瘤/白血病EL4细胞/细胞增殖/细胞表面共刺激分子Key words
decitabine/lymphoma EL4 cells/proliferation/inhibition/costimulatory molecules分类
医药卫生引用本文复制引用
王立新,丁洁,陈晓平,胡小山,姚雨石..国产地西他滨对小鼠淋巴瘤／白血病细胞EL4形态、增殖及CD80表达的影响[J].山东医药,2014,(38):5-7,3.基金项目
国家自然科学基金资助项目（81350004）；海军总医院创新培育基金项目（cxpy201302）。 （）
