山东医药Issue(38)：26-28,3.DOI:10.3969/j.issn.1002-266X.2014.38.008
骨髓间充质干细胞移植对大鼠重症急性胰腺炎的治疗作用观察
Treatment effect of bone marrow mesenchymal stem cells transplantation on the severe acute pancreatitis in rats
摘要
Abstract
Objective To study the protective effect and the possible mechanisms of transplantation bone marrow mes-enchymal stem cells (MSCs) on pancreatic injury in rats with severe acute pancreatitis(SAP).Methods A total of 120 male Sprague-Dawley rats were randomly divided into sham operation group( N group) , severe acute pancreatitis group ( P group) , transplantation MSCs group( M group) .The dose of 5%sodium taurocholate was retrograde injected into the pan-creatic duct of the rats in P group and M group to induce SAP model.Rats in N group whose pancreases were just flipped and striked gently without injection.MSCs were transplanted into rats in M group via portal vein, while the same dosage of 0.9%NS was injected by the same vein routine in P group.Ten rats in each group were sacrificed at 24 h, 48 h, 72 h lat-er.Pancreas and kidney damage of each group were observed under gross morphology and optical lens.The serum AMY, BUN, Cr, TNF-αand IL-6 level of each group were tested.Results Pancreas and kidney injury in P group were signifi-cantly heavier.The content of serum AMY, BUN, Cr, TNF-αand IL-6 level in P group were both higher than those of N group(P<0.05).Pancreatic and renal tissue injury in M group rats mitigated.The content of serum AMY, BUN, Cr, TNF-αand IL-6 level in M group were both lower than those of P group (P<0.05).Conclusions Transplantation via portal vein MSCs is an effective treatment on SAP, which can reduce the occurrence of multiple organ dysfunction and im-prove prognosis.关键词
骨髓间充质干细胞,细胞移植/重症急性胰腺炎Key words
bone marrow mesenchymal stem cells/cell transplantation/severe acute pancreatitis分类
医药卫生引用本文复制引用
王忠琼,刘翼,夏国栋,杨春,钟晓琳..骨髓间充质干细胞移植对大鼠重症急性胰腺炎的治疗作用观察[J].山东医药,2014,(38):26-28,3.基金项目
四川省卫生厅科研基金资助项目（070267）。 （）
