山东医药Issue(38)：29-31,3.DOI:10.3969/j.issn.1002-266X.2014.38.009
短期能量限制对正常大鼠胰岛素敏感性的影响
Effects of short-term caloric restriction on insulin sensitivity in normal rats
摘要
Abstract
Objective To investigate the effect of the short-term caloric restriction( CR) on insulin sensitivity and its signal transduction mechanisms.Methods A total of 24 F344/NSIc rats were equally divided into ad libitum group ( AL group) and CR group.Rats in AL group were free fed every alternate day for 8 weeks, while rats in CR group were fed re-strict caloric without water deprivation for 8 weeks.Glucose infusion rate(GIR) and insulin sensitivity were measured by u-sing hyperinsulinemic-euglycemic clamp method.The body weight mass and visceral fat weight between the two groups were compared.The muscle glucose transporter 4(GluT4), kinase substrate 160(AS160 ) protein and p-AS160 protein was de-tected by using Western blot method.Results GIR in CR group was obviously higher than that of control group, while body mass and visceral fat weight significantly lower than control group.However, there was no significant difference in ex-pression of GluT4, AS160 protein and p-AS160 protein.Conclusions Short-term caloric restriction can improve the insu-lin sensitivity in healthy rats which may be associated with reduced the visceral fat increasing.关键词
能量限制/胰岛素敏感性/葡萄糖转运体4/蛋白激酶B底物蛋白160Key words
caloric restriction/insulin sensitivity/glucose transporter 4/kinase substrate 160分类
医药卫生引用本文复制引用
朴雄,卢焕俊,李香兰,姜海英..短期能量限制对正常大鼠胰岛素敏感性的影响[J].山东医药,2014,(38):29-31,3.基金项目
吉林省卫生厅科研计划项目（2013Z071）。 （）
