山东医药Issue(40)：4-6,9,4.DOI:10.3969/j.issn.1002-266X.2014.40.002
脓毒症大鼠肝脏组织中免疫相关基因表达的改变
Changes of immune related gene-expression profiling in rats with sepsis
摘要
Abstract
Objective To observe the change of immune related gene-expression profiling in rats with sepsis.Meth-ods 120 male wistar rats were randomly divided into sham operation group ( n=30 ) and model group ( n=90 ) .The model group according to different time points (24 h, 48 h and 72 h) were randomly divided into 3 groups ( each subgroup n=30).The rats of model group received cecal ligature and puncture (CLP) operation.Gene chips containing 28 000 rot-genes cDNA were used to test gene-expression in liver of septic rats to select the genes with different expressions with soft-ware.Results Model group had 671 differential expression genes with 97 immune related genes at 24 h;133 differential expression genes with 36 immune related genes at 48 h;204 differential expression genes with 33 immune related genes at 72 h.Three time points in model group and sham operation group repeated gene were 58, immune related gene 15.Con-clusion A series of cell defense and immune function genes change significantly in liver tissue of septic rats, which may be involved in the pathophysiology of sepsis induced hepatic injury in different extent.关键词
脓毒症/基因芯片/基因表达/盲肠结扎穿孔术/大鼠Key words
sepsis/gene chip/gene expression/cecal ligation and perforation/rat分类
医药卫生引用本文复制引用
毛莹莹,王东强,李志军..脓毒症大鼠肝脏组织中免疫相关基因表达的改变[J].山东医药,2014,(40):4-6,9,4.基金项目
天津市自然科学基金项目（12JCYBJC17600）。 （）
