山东医药Issue(40)：10-12,15,4.DOI:10.3969/j.issn.1002-266X.2014.40.004
PPAR-γ对人鼻咽癌 CNE1／DDP 细胞顺铂耐药性的逆转作用及其机制探讨
Effect and mechanism of PPAR-γon cisplatin resistance reversal of human nasopharyngeal carcinoma CNE1/DDP cells
陈磊 1郑树艳 2黄永望1
作者信息
- 1. 天津医科大学第二医院，天津300211
- 2. 天津市咸水沽医院
- 折叠
摘要
Abstract
Objective To investigate the effect of PPAR-γon cisplatin sensitivity reversal of human nasopharyngeal carcinoma CNE1/DDP cell line and its mechanism.Methods The human nasopharyngeal carcinoma CNE1/DDP cells were treated by 10 μg/mL and 20 μg/mL PPAR-γagonist rosiglitazone.MTS assay was used to measure the effect of pro-liferation;flow cytometry was used to measure the cell apoptosis and P-gp expression;RT-PCR and Western blot assay were used to measure the mRNA and protein expression of multidrug resistance gene (MDR1), multidrug resistance-associated protein ( MRP) , B-cell lymphoma gene 2 ( bcl-2);Western blot assay was used to measure the phosphorylation of Akt ( p-Akt).Results CNE1/DDP cells were treated by 10μg/mL and 20μg/mL rosiglitazone, the drug resistance reverse rates were 100.0%, 149.3%±11.3%, 293.8%±25.5%, respectively;the apoptosis rate was gradually increased;the mR-NA and protein expression of PPAR-γ, MDR1, MRP and bcl-2, and p-Akt expression were gradually decreased (all P<0.05).Conclusions PPAR-γcould increase cisplatin sensitivity of CNE1/DDP cells by down-regulating phosphorylation of Akt and the expression of MDR1 and MRP.关键词
鼻咽癌/氧化物酶体增殖物激活受体-γ/CNE1/DDP细胞/顺铂/耐药基因/细胞凋亡Key words
nasopharyngeal carcinoma/PPAR-γ/CNE1/DDP cell/cisplatin/drug resistance gene/apoptosis分类
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陈磊,郑树艳,黄永望..PPAR-γ对人鼻咽癌 CNE1／DDP 细胞顺铂耐药性的逆转作用及其机制探讨[J].山东医药,2014,(40):10-12,15,4.
