山东医药Issue(42)：17-19,3.DOI:10.3969/j.issn.1002-266X.2014.42.006
糖尿病患者脂肪间充质干细胞的分离培养与鉴定
Culture and identification of adipose mesenchymal stem cells from diabetic patients in vitro
摘要
Abstract
Objective To establish the isolation and culture method of adipose mesenchymal stem cells derived from diabetic patients in vitro, so as to observe biological properties.Methods The ADSCs were isolated by digestion with col-lagenase from adipose tissue of diabetic patients, then cultured and purified in vitro.The cells were observed by inverted microscopeand.The P3, P6, P8 generation cells were collected and analyzed the cell growth curve.Then the cell surface markers and karyotype analysis of the P3 generation cells were detected.Their differentiation potential was proved by adipo-genic differentiation.Oil red staining positive proved they were fat cells.Results After isolation and culture, we obtained the ADSCs, which could passage stably and proliferate highly.The flow cytometry examination showed that the expression of CD34 and CD45 were negative, CD29 , CD90 , CD44 and CD105 were positive.Oil red staining was positive.Conclusion The ADSCs could be isolated from diabetic patients adipose tissue and be functionally induced into adipocytes in the pres-ence of appropriate conditioned media.关键词
糖尿病/脂肪间充质干细胞/分化潜能Key words
diabetes mellitus/adipose tissue-derived stromal cells/differentiation potential分类
医药卫生引用本文复制引用
拾莉,张德明,任思坡,韩光宇,谭昆,耿跃春,梁军..糖尿病患者脂肪间充质干细胞的分离培养与鉴定[J].山东医药,2014,(42):17-19,3.基金项目
徐州市科技发展基金计划项目（XZZD1045，XZZD1142）。 （）
