山东医药Issue(43)：31-33,3.DOI:10.3969/j.issn.1002-266X.2014.43.010
法舒地尔预处理对大鼠缺血再灌注损伤肝脏的保护作用及机制探讨
The protective effect and mechanism of Fasudil preconditioning on hepatic ischemia-reperfusion injury in rats
摘要
Abstract
Objective To investigate the protective effect and mechanism of Fasudil preconditioning on hepaitc ische -mia-reperfusion injury in rats .Methods 30 adult SD rats were randomly divided into sham group ( S group ) , ischemia-reperfusion group ( IR group:produced by total inflow occlusion for 30 minutes) , fasudil group ( FAS group:fasudil was in-traperitoneal injected 30 minutes before ischemia ) , 10 rats in each group .The hepatic artery was completely block with no damage clamp for 30 minutes in IR group and FAS group, and the clamp was removed 2 hours later.In FAS group, fasudil was intraperitoneal injected with the dose of 10 mg/kg before the artery was blocked , and in IR group , saline was intraper-itoneal injected with the same dose .In S group, we just dissected the hepatic ligaments and exposed hepatic hilar vessels without blocking blood flow .After the models had been established , serum ALT, AST and the liver tissue superoxide dis-mutase (SOD) activity, malondialdehyde (MDA) content and myeloperoxidase(MPO) activity were measured after reper-fusion for 2 hours.And the liver pathological changes were observed .Results Compared with the S group , the serum ALT, AST levels in IR group and FAS group were significantly higher (all P<0.05).Compared with IR group, the serum ALT, AST levels in FAS group were lower (all P<0.05).Compared with the S group, IR group and FAS group SOD ac-tivity decreased (all P<0.05), MDA content and MPO activity increased (all P<0.05).Compared with IR group, FAS group SOD activity increased (P<0.05), MDA content and MPO activity decreased (all P<0.05).For the pathological changes of the liver , the hepatic cords distributed orderly , and the liver cell morphology was normal in S group .After is-chemia-reperfusion for 2 h, the hepatic cell cord became disorder , and the liver cells were swelling , with vacuolar degener-ation, and the alignment of sinusoidal space narrowed or disappeared , the inflammatory cells were infiltrating in IR group . Compared with IR group ,the disorderness of hepatic cords , swelling of liver cells were reduced in FAS group , and the infil-tration of inflammatory cell were less in FAS group .Conclusion Effect of Fasudil preconditioning on ischemia-reperfusion injury of the liver is protective and its mechanism may involve the improvement of the body 's resistance to oxidative stress and reduce neutrophilic infiltration in liver tissues .关键词
法舒地尔/缺血预处理/再灌注损伤/肝脏损伤/肝脏缺血再灌注损伤Key words
Fasudil/ischemic preconditioning/reperfusion injury/liver damage/hepatic ischemia-reperfusion injury分类
医药卫生引用本文复制引用
洪晓鹏,朱耿隆,贾英斌,林志东,张百萌..法舒地尔预处理对大鼠缺血再灌注损伤肝脏的保护作用及机制探讨[J].山东医药,2014,(43):31-33,3.基金项目
广东省医学科研基金资助项目（A2012209）。 （）
