山东医药Issue(44)：29-31,3.DOI:10.3969/j.issn.1002-266X.2014.44.011
肺癌细胞与组织中miR-100、miR-148a的表达变化及临床意义
Expression and clinical significance of miR-100 and miR-148a in lung cancer cells and tissues
摘要
Abstract
Objective To investigate the expression and clinical significance of miR-100 and miR-148a in lung cancer cells and tissues.Methods RT-PCR method was used to detect the expression of miR-100 and miR-148a in lung cancer cell lines NCI-H1299, NCI-H358, H460, A549 and normal human bronchial epithelial cell line HBE, and in the lung cancer tissues and adjacent tissues.To analyze the relationship between the expression of miR-100 and miR-148a and clini-copathological parameters of lung cancer.Results Compared with HBE cells, miR-100 expression in NCI-H1299, NCI-H358 and A549 increased significantly, decreased in H460, all P<0.05; the expression of miR-148a in NCI-H358 and H460 increased, decreased in A549, all P<0.05.Compared to non-tumor tissues, miR-100 expression was reduced in lung cancer, but the difference was not statistically significant (P=0.400);while the expression of miR-148a was signifi-cantly increased (P=0.036).miR-100 expression in lung cancer tissue with lymph node metastasis was lower than that in lung cancer tissue without lymph node metastasis (P=0.016).Conclusions The expression of miR-148a, miR-100 were aberrantly changed in lung cancer cells.miR-100 expression decreased in lung cancer tissues, miR-148a increased, which both maight be involved in the tumorigenesis of lung cancer.关键词
肺肿瘤/微小RNA 100/微小RNA 148a/转移Key words
lung cancer/miR-10/miR-148a/metastasis分类
医药卫生引用本文复制引用
王勇,李宇男,于晓松..肺癌细胞与组织中miR-100、miR-148a的表达变化及临床意义[J].山东医药,2014,(44):29-31,3.基金项目
国家自然科学基金面上项目（71273279）。 （）
