山东医药Issue(44)：40-42,3.DOI:10.3969/j.issn.1002-266X.2014.44.015
瘦素对快速动眼睡眠剥夺大鼠焦虑行为的影响及其机制探讨
Effects of leptin on the anxiety behavior in REM sleep deprivated rats and its mechanisms
摘要
Abstract
Objective To observe the effects of leptin on the anxiety behavior in REM sleep deprived rats, and to ex-plore its mechanisms.Methods A total of 90 male Wistar rats were randomly divided into 9 groups on average, as fol-lows:living together in home cage ( NS group) for 24, 72 and 120 h with NS,REM sleep deprived for 24,72 and 120 h with leptin ( L-SD group) or with NS ( NS-SD group) .The single platform ( SP) method was used to build the REM sleep deprivation model;the elevated plus-maze ( EPM) was used to assess the anxiety behavior;the RT-PCR method was used to measure the level of neuropeptide Y ( NPY) mRNA.Results At 24 h and 120 h, numbers of open arm ( OE) +closed arm (CE) in NS-SD and L-SD groups was higher than that in NS group (all P<0.01);the difference of OE+CE among 3 groups was not significant (P=0.47).At 120 h, compared with NS, OE ratio (OE%) and stay open arm ratio (OT%) increased in NS-SD group, but decreased in L-SD group, all P<0.01.At 120 h time point, OE%and OT%in NS-SD group were higher than those in 24 h and 72 h;but OE%and OT%at 120 h in L-SD group were lower than those in 24 h and 72 h;all P<0.01.Compared with NS group at the same time, NPY mRNA level in hypothalamus in NS-SD and L-SD groups increased (P<0.05 or P<0.01).At 24 h, 72 h, 120 h, NPY mRNA in NS-SD group increased gradu-ally, which in L-SD group decreased gradually, all P<0.05.Conclusion Chronic REM sleep deprivation could decrease the anxiety behavior in rats, but leptin could eliminate this effect;leptin inhibited the expression of NPY in hypothalamus might be one of its mechanisms.关键词
睡眠剥夺/瘦素/神经肽Y/快速动眼/焦虑Key words
sleep deprivation/leptin/neuropeptide Y/rapid eye movement/anxiety分类
医药卫生引用本文复制引用
马文彬,韩笑峰,郑麒,陈芳,李希芝,葛汝丽..瘦素对快速动眼睡眠剥夺大鼠焦虑行为的影响及其机制探讨[J].山东医药,2014,(44):40-42,3.基金项目
滨州医学院科技计划项目（ BY2011 KJ002）。 （）
