山东医药Issue(45)：1-3,3.DOI:10.3969/j.issn.1002-266X.2014.45.001
缺氧缺血性脑损伤大鼠脑组织海马区 p-tau 蛋白与凋亡相关蛋白 Bcl-2、Bax 的关系
The connection among the expression of p-tau protein and apoptosis related proteins Bcl-2 and Bax in the hippocampus of hypoxic-ischemic neonatal rats
摘要
Abstract
Objective To observe the expression of phosphorylated tau ( p-tau) protein, and apoptosis related pro-teins Bcl-2 and Bax, and explore their correlations .Methods Seven-day-old newborn clean Sprague Dawley ( SD) rats, were randomly divided into normal control group (8 cases), sham operation (8 cases), and HIBD group (which was divid-ed into by death time points 3, 6, 12, 24, 48 h subsection 8 cases in each group).HIBD animal model was established by Rice method, and took the brain at different time points .In sham group, rats were just exposed the left carotid artery , and in the control group rats did not dispose .Rats were all decapitated brain immediately at the end of surgery .Hematoxylin-e-osin ( HE) staining was used to view morphological changes in the hippocampus , and immunohistochemical staining was used to test the expressions of p-tau, Bcl-2 and Bax protein.Results Brain tissues in the normal control group and sham operation group was normal .In HIBD group, the cell has not significant change 3 h after HIBD;Cell swelling and structur-al disorder 6 h after HIBD; Cell swelling significantly , nuclear condensation , fragmentation 24 h after HIBD;48 h after HIBD, apoptotic bodies occured , the number of nerve cells was dropped .In HIBD group, Bcl-2 and Bax positive cells in HIBD group at each time point were more than those in the normal control group and the sham group (all P<0.05);In the hippocampus of HIBD group , p-tau positive cells was positively correlated with Bcl-2 and Bax positive cells during 12 h af-ter HIBD(P<0.05).Conclusion The hippocampus neurolcytes actiwated apotosis mechanism afer HIBD early period . P-tau protein was involved in neuronal apoptosis after HIBD , and there is connection among p-tau, Bcl-2 and Bax protein.关键词
缺氧缺血,脑/p-tau蛋白/细胞凋亡/Bcl-2/Bax/大鼠Key words
hypoxic-ischemia, brain/p-tau protein/apoptosis/Bcl-2/Bax/rat分类
医药卫生引用本文复制引用
王军,陈静,张冬秀,王宝珍,朱登纳,牛国辉,程萍萍..缺氧缺血性脑损伤大鼠脑组织海马区 p-tau 蛋白与凋亡相关蛋白 Bcl-2、Bax 的关系[J].山东医药,2014,(45):1-3,3.基金项目
河南省教育厅科学技术研究重点项目（14A320004）。 （）
