山东医药Issue(46)：21-23,3.DOI:10.3969/j.issn.1002-266X.2014.46.007
气化过氧化氢用于生物安全实验室消毒最佳浓度及剂量探讨
Design of the best dosage of vapor hydrogen peroxide in disinfection of biosafety laboratory
摘要
Abstract
Objective To design the best dosage of vapor hydrogen peroxide in disinfection of high pathogenic micro -organism biosafety laboratory .Methods One room of 120 m3 in biosafety laboratory was selected .Hydrogen peroxide solu-tion was vaporized by hydrogen peroxide sterilizer ( VHP) .Nine types of vapor hydrogen peroxide inject speed and duration time were used to disinfect .After the disinfection , the chemical indicators and biological indicators were placed on 10 loca-tions in the room to observe the color of chemical indicators and the growth condition of all the plates inoculated with Bacil -lus subtilis var niger .After the 9 types of disinfection , the minimum vapor hydrogen peroxide was determined and then re-peated for 6 times in order to verify the disinfection effect .Results Qualified disinfection was proved that the color of the chemical indicators changed from orchid to ecru when the dosage no less than 5 g/M3 .The minimum vapor hydrogen perox-ide was determined at 5 g/m3 .After 6 times repeation of disinfection experiment at 5 g/m3 , all the disinfection results were qualified.Conclusions The best dosage of hydrogen peroxide solution with concentration of 35% was 5 g/m3 .It can reach the good efficacy in disinfection of air and surfaces in biosafety laboratory , which was suit for the disinfection in high pathogenic microorganism biosafety laboratory .关键词
生物安全实验室/过氧化氢/消毒/最佳剂量Key words
biosafety laboratory/hydrogen peroxide/disinfection/best dosage分类
医药卫生引用本文复制引用
孙蓓,赵四清,李纲,陈梅玲,靳晓军,周康力..气化过氧化氢用于生物安全实验室消毒最佳浓度及剂量探讨[J].山东医药,2014,(46):21-23,3.基金项目
国家高技术研究发展计划863计划（2012AA022004）。 （）
