山东医药Issue(47)：17-20,4.DOI:10.3969/j.issn.1002-266X.2014.47.005
生长因子分步诱导法在大鼠脂肪干细胞向神经细胞分化中的应用观察
The observation on application of growth factor two-step method for inducing adipose stem cells into nerve cells in rat
摘要
Abstract
Objective To investigate the effect of rat adipose tissue-derived cells ( ADSCs) into neural cells with a growth factor two-step induction method .Methods ADSCs were isolated from the fat pads in inguinal and retroperitoneal of Sprague Dawley rats , and the third generation cells were cultured .First, the cells were induced with DMEM/F12+2%B27+20 ng/mL EGF+20 ng/mL bFGF for 7 days, and then DMEM/F12+1μmol/L RA for another 7 days.Immunocyto-chemistry was taked to identify the expression of the neuron specific markers Nestin , MAP2, NeuN and GFAP.Results ADSCs were reaped from rat fat tissue , and could passage in a considerable large amount .ADSCs could strongly express CD73(99.17%), CD90(98.61%), CD105(96.20%)and lowy expressed CD34(0.36%).The ADSCs induced for 7 days aggregated as floating cell spheres and positive for Nestin , then strongly express MAP2 and NeuN, and lowy expressed GFAP after 14 days.Conclusion The growth factor two-step induction method can efficiently induce ADSCs to differenti-ate into nerve cells .关键词
生长因子/脂肪干细胞/神经细胞Key words
growth factor/adipose stem cells/nerve cells分类
医药卫生引用本文复制引用
周六杰,夏英鹏..生长因子分步诱导法在大鼠脂肪干细胞向神经细胞分化中的应用观察[J].山东医药,2014,(47):17-20,4.基金项目
新团队科学基金资助项目（WHTD201306）。 （）
