山东医药Issue(3)：8-10,3.DOI:10.3969/j.issn.1002-266X.2015.03.003
高危型人乳头瘤病毒阳性的宫颈癌组织中L1基因甲基化水平变化及意义
The methylation level changes of the L1 gene in high-risk human papillomavirus positive cervical cancer tissue and its significance
摘要
Abstract
Objective To observe the changes of methylation level of the long interspersed nucleotid element -1(L1)in persistent high-risk human papillomavirus ( HR-HPV) positive cervical cancer tissue and its significance .Methods Thirty tissue samples were collected in all HR-HPV infected normal cervix , cervical intraepithelial neoplasia ( CIN)Ⅰ, CINⅡ-CINⅢand cervical cancer respectively .Three CpG sites methylation levels of the L 1 of all tissues were quantitatively ana-lyzed by bisulphite conversion and pyrosequencing technology .Evaluated its diagnostic value in cervical cancer and CIN . Results The mean levels and standard deviation of the L 1 promoter methylation level of normal cervix tissue , CINⅠtis-sue, CINⅡ~Ⅲtissue and cervical cancer tissue were 0.59 ±0.053, 0.57 ±0.038, 0.56 ±0.025, 0.49 ±0.054 re-spectively.Compared the L1 promoter mean methylation level of cervical cancer tissues with other tissues , all P<0.001. Selected the cutoff value of the L 1 gene methylation level as 53 .17%, both the sensitivity and specificity to judge the prop-erty of cervical lesions were 86.70%, with the 95%confidence interval (CI) was 0.834~0.976.Conclusions The L1 gene methylation level of HR-HPV positive cervical cancer tissue decrease significantly .It helps to judge the property of cervical lesions with the L1 gene promoter methylation level of 53.17%.关键词
子宫肿瘤/宫颈癌/长散布核元件-1/DNA甲基化Key words
cervical lesions/cervical cancer/long interspersed nucleotid element-1/DNA methylation分类
医药卫生引用本文复制引用
付玉娇,胡元晶..高危型人乳头瘤病毒阳性的宫颈癌组织中L1基因甲基化水平变化及意义[J].山东医药,2015,(3):8-10,3.基金项目
天津市卫生局科技基金资助项目（13KG134）。 （）
