山东医药Issue(5)：22-24,27,4.DOI:10.3969/j.issn.1002-266X.2015.05.008
槲皮素对缺血再灌注大鼠肝脏损伤的保护作用
Protection of quercetin against liver ischemia reperfusion injury in rats
摘要
Abstract
Objective To establish rat ischemia reperfusion injury model and evaluate the protection effect of querce -tin against liver ischemia reperfusion injury in rats .Methods A total of 80 healthy rats were randomly divided into control group(n=5), sham group(n=25), ischemia reperfusion injury group(n=25) and quercetin group(n=25).The later three groups were divided into 0, 6, 12, 24, 72 h subgroups(n=5), respectively.At the end of the experiment, the blood samples and liver tissues were collected .The levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αwere determined by automatic biochemistry analyzer and competitive inhibition ELISA , respectively .The morphology change of liver were de-tected by HE staining, and the apoptosis of liver cells were detected by TUNEL .Results In 0, 6, 12, 24, 72 h sub-group, the levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αincreased, especially in 12 h group, which had significant statistically difference compared with control or sham group (all P<0.05).Under light microscope, there was no signifi-cant change in control or sham group .In ischemia reperfusion injury group , liver cells degenerated significantly , liver cord structure disappeared, some liver cells necrosed.However, in quercetin group, only part liver cells degenerated , part liver cord structure exsited , which was consistent with the results of apoptosis index .Conclusion Quercetin is beneficial for liver ischemia reperfusion injury .关键词
槲皮素/缺血再灌注损伤/凋亡/炎症因子/大鼠Key words
quercetin/ischemia reperfusion injury/apoptosis/inflammatory factors/rats分类
医药卫生引用本文复制引用
王甲甲,钟佳伶,张娟,黄文芳..槲皮素对缺血再灌注大鼠肝脏损伤的保护作用[J].山东医药,2015,(5):22-24,27,4.基金项目
四川省人民医院博士基金资助项目（30305030569）。 （）
