山东医药Issue(8)：7-10,4.DOI:10.3969/j.issn.1002-266X.2015.08.003
大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数探讨
Discussion of the optimum conditions and parameters of separation and purification of total flavones from Malus doumeri (Bois) Chev by macroreticular resin
摘要
Abstract
Objective To explore the optimum technological parameters of the separation and purification process of total flavones from Malus doumeri (Bois) Chev with macroreticular resins.Methods With the static and dynamic saturat-ed adsorption and elution rate as index , compare the advantages of nonpolar resin D 101 , weak polarity resin AB-8, medium polarity resin HPD-450 used in isolation and purification of total flavones from Malus doumeri (Bois) Chev, and optimize the adsorption process parameters of selected optimum resin by investigating the total flavones recovery rate .Results The static saturated adsorption of D 101 , AB-8, HPD-450 were 94.802, 118.494, 98.317 mg/g dry resin, the static elution rate were 83.434%, 91.253%and 83.434%respectively;the amount of total flavones eluted were 91.490, 115.567, 98.528 mg, elution rate were 85.852%, 93.217%and 89.051%respectively.Eluted with five times resin volume of 70%etha-nol, flow rate of 1.5 mL/min, the recovery rate of total flavones was 94.09%, the purity of which was 73.77%.Conclu-sion The comprehensive performance of AB-8 resin was the best , the optimum process parameters were that of eluted with five times resin volume of 70%ethanol and 1.5 mL/min flow rate.关键词
广山楂总黄酮/大孔吸附树脂/分离纯化Key words
total flavones of Malus doumeri ( Bois) Chev/macroreticular resin/separation and purification分类
医药卫生引用本文复制引用
陈洪涛,刘源焕,覃学谦,蔡丹昭..大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数探讨[J].山东医药,2015,(8):7-10,4.基金项目
国家自然科学基金资助项目（81360688）；广西自然科学基金资助项目（2012GXNSFAA239008）；广西壮族自治区卫生厅中医药民族医药自筹经费课题项目（gzzc1203）；广西壮族自治区教育厅广西高等学校科研项目（201204LX195）。 （）
