山东医药Issue(10)：1-3,3.DOI:10.3969/j.issn.1002-266X.2015.10.001
丹参多酚对铁过载引起的脐血造血细胞氧化应激损伤的抑制作用
Inhibitory effect of salvianolate on iron overload-derived oxidative stress injury of hematopoietic cells of umbilical cord blood
摘要
Abstract
Objective To investigate the protective effect of salvianolate on the iron overload-derived oxidative stress injury of mononuclear cells of umbilical cord blood (UC-MNCs).Methods The UC-MNCs were divided into the control group, FAC group, low-dose salvianolate group, medium-dose salvianolate group, and high-dose salvianolate group.The control group received normal culture .The iron overload cell models in the FAC and salvianolate groups were established by adding 400 μmol/L FAC into the culture medium for 24 h on the second day.Then the low-dose, medium-dose and high-dose salvianolate groups were received 18, 50 and 160μg/mL salvianolate for 24 h, respectively on the third day .Then the levels of reactive oxygen species ( ROS) , apoptosis rate and LIP level were detected by flow cytometry , respectively .Re-sults The ROS level of the FAC group was higher than that of the control , the low-dose, medium-dose and high-dose salvianolate groups (all P<0.05).The apoptosis rate in the FAC group was higher than that of the control group and high-dose salvianolate group(P<0.05).The counts of colony-forming unit-erythroid (CFU-E), granulocyte-macrophage colo-ny-forming units ( CFU-GM) , erythropoietic burst-forming units ( BFU-E) and mixed progenitor cell colony-forming units ( CFU-mix) in the FAC group were significantly lower than those of the control group and high-dose salvianolate group ( P<0.05).Conclusions Salvianolate can reduce the oxidative stress caused by apoptosis and inhibition of proliferation and differentiation of hematopoietic cells .关键词
丹参多酚/铁过载/氧化应激/细胞凋亡/造血干细胞/血液病Key words
salvianolate/iron overload/oxidative stress/apoptosis/hematopoietic stem cells/hematologic diseases分类
医药卫生引用本文复制引用
穆娟,赵明峰,李玉明,张宇辰,曹小立,李青..丹参多酚对铁过载引起的脐血造血细胞氧化应激损伤的抑制作用[J].山东医药,2015,(10):1-3,3.基金项目
天津市自然科学基金资助项目（13JCYBJC23400）；天津市中医药管理局中西医结合基金资助项目（13120）；天津市卫生局科技基金重点资助项目（2011KR01）。 （）
