山东医药Issue(15)：4-7,4.DOI:10.3969/j.issn.1002-266X.2015.015.002
冬凌草甲素纳米结晶的制备及其体外对结肠癌细胞增殖与凋亡的影响
Preparation of nano-crystalline of oridonin and its effect on cell proliferation and apoptosis of colorectal cancer cells in vitro
摘要
Abstract
Objective To prepare the nano-crystalline of oridonin and to investigate the effect on the proliferation and apoptosis of colorectal cancer cells.Methods The contents of oridonin nanosuspensions were prepared by high pressure homogenization.The nano-crystalline of oridonin was obtained after adding 5% mannitol, and then shaking and lyophili-zing.The solubility was determined in vitro.Colorectal cancer cells ( Lovo cells, SW480 cells, HCT-116 cells and HCT-8 cells) were cultured in vitro and incubated with different concentrations of nano-crystalline of oridonin (0, 5, 10, 15 and 25 μg/mL) .Cell proliferation was detected by cytometry.The apoptosis and cell cycle of cultured cells in different concen-trations of nano-crystalline of oridonin (5, 10 and 25 μg/mL) were detected.Results The nanosuspensions and nano-crystalline of oridonin was prepared successfully.There were significant differences in the proliferation inhibition rate among four kinds of colorectal cancer cells that were intervened by different concentrations of nano-crystalline of oridonin for 24 h and 72 h ( all P<0.05 ) .The nano-crystalline of oridonin with concentration of 25 μg/mL had the strongest inhibitory effect on cell proliferation as compared with the others (all P<0.05).The nano-crystalline of oridonin could block cell cy-cle of the Lovo cells and HCT-8 cells at G1 phase, and block the HCT-16 and SW480 cell cycle at S phase.Conclusion The vitro dissolution of nano-crystalline of oridonin is higher than oridonin.The nano-crystalline of oridonin can significant-ly inhibit the proliferation, induce the apoptosis and block the cell cycle of four kinds of colorectal cancer cells.关键词
冬凌草甲素/冬凌草甲素纳米结晶/人结肠癌细胞/细胞增殖/细胞凋亡/细胞周期/体外试验Key words
oridonin/nano-crystalline of oridonin/human colorectal cancer cells/cell proliferation/apoptosis/cell cycle/in vitro experiment分类
医药卫生引用本文复制引用
王永强,金黑鹰,李丽梅,王水明,吴闯..冬凌草甲素纳米结晶的制备及其体外对结肠癌细胞增殖与凋亡的影响[J].山东医药,2015,(15):4-7,4.基金项目
昆山市社会发展科技计划项目（KS1129）。 （）
