山东医药Issue(15)：17-19,3.DOI:10.3969/j.issn.1002-266X.2015.015.006
小鼠睾丸组织中核受体4A1的表达变化及体外对睾丸间质细胞睾酮合成的影响
Expression changes of nuclear receptor 4 A1 in mouse testis and its effect on testosterone synthesis of Leydig cells in vitro
摘要
Abstract
Objective To observe the expression changes of nuclear receptor ( NR) 4A1 in mouse testis and its effect on testosterone synthesis of Leydig cells in vitro, and to explore its mechanism.Methods The male mice of 1 week old, 4 weeks old, 12 weeks old and 12 months old were respectively divided into the infancy group, before sexual development group, sexual maturity group and the older group with 6 mice in each.After being fed for three days and adapting to the en-vironment, all the mice in each group were sacrificed.We took the fresh bilateral testicular tissue to detect NR4A1 mRNA by using real-time PCR, and NR4A1 protein by using Western blotting.We cultured the mouse Leydig cells MLTC-1 in vitro, and then used NR4A1 interference adenovirus ( Ad-siNR4A1 ) and no-load adenovirus ( Ad-CMV ) to transfect MLTC-1.The radioimmunoassay was used to measure the testosterone level in medium.real-time PCR and Western blotting were applied to determine the mRNA and protein expression of steroidogenic acute regulatory protein ( StAR ) and 3β-hydroxysteroid dehydrogenase (HSD3B).Results The NR4A1 mRNA expression was the highest in the before sexual de-velopment group among the infancy group, before sexual development group, sexual maturity group and the older group;and significant difference was found as compared with the other three groups (all P<0.05).NR4A1 protein expression was the highest in the sexual maturity group as compared with that of the other three groups ( all P<0.05) .The testoster-one levels in MLTC-1 transfected with Ad-siNR4A1 or Ad-CMV were respectively (2.55 ±0.23), (4.50 ±0.23) nmol/L (all P<0.05).The StAR and HSD3B mRNA and protein expression levels in the MLTC-1 transfected with Ad-siNR4A1 were relatively low as compared with those in the Ad-CMV-transfected MLTC-1 (all P<0.05).Conclusion NR4A1 ex-pression is different in testis tissues of mice with different age.NR4A1 is involved in the synthesis of testosterone possibly by regulating the expression of StAR and HSD3B.关键词
核受体4A1/睾丸/睾丸间质细胞/睾酮/3β-羟类固醇脱氢酶/类固醇快速转运蛋白Key words
nuclear receptor 4A1/testis/Leydig cells/testosterone/3β-hydroxysteroid dehydrogenase/steroidogenic acute regulatory protein分类
医药卫生引用本文复制引用
谷亚龙,张新东,金保方..小鼠睾丸组织中核受体4A1的表达变化及体外对睾丸间质细胞睾酮合成的影响[J].山东医药,2015,(15):17-19,3.基金项目
国家自然科学基金青年基金资助项目（81302969）；国家自然科学基金资助项目（81273760）；国家自然科学基金资助项目（81473678）；南京中医药大学青年自然科学基金项目（12XZR13）。 （）
