山东医药Issue(16)：8-11,4.DOI:10.3969/j.issn.1002-266X.2015.16.003
姜黄素-甘草次酸-PEI-PLGA 纳米粒的制备及工艺优化
Preparation of curcumin-glycyrrhetinic acid-PEI-PLGA nanoparticles and the process optimization
摘要
Abstract
Objective To prepare curcumin (Cur)-glycyrrhetinic acid (GA)-PEI-PLGA nanoparticles and optimize the preparation process .Methods Cur-GA-PEI-PLGA nanoparticles were prepared by thin film hydration method .After establishing the analysis method , single factor experiment and orthogonal design experiment were used to evaluate the effects of hydration temperature , hydration time and drug addition on the entrapment efficiency and drug loading , and to determine the optimum preparation conditions .Cur-GA-PEI-PLGA nanoparticles were prepared under the optimum preparation condi-tions , and we determined the entrapment efficiency and drug loading .The morphology of the nanoparticles was observed by transmission electron microscope (TEM).The diameter and zeta potential of the nanoparticles were determined by dynamic light scattering particle size analyzer ( DLS) .Results The optimum preparation conditions of Cur-GA-PEI-PLGA nanopar-ticles were as follows:the hydration temperature was 60 ℃, the hydration time was 3 h, and the drug addition was 7 mg. The entrapment efficiency and drug loading were (58.1 ±1.2)%and (14.5 ±1.4)%under these conditions .The nanop-articles were spherical and uniform under TEM .The average particle size was 330.3 nm, and the nanoparticles distributed evenly (PDI was 0.311), zeta potential was 39.2 mV.Conclusions Thin film hydration method is suitable for the prepa-ration of Cur-GA-PEI-PLGA nanoparticles .The optimum preparation conditions are as follows:the hydration temperature is 60 ℃, the hydration time is 3 h, and the drug addition is 7 mg.关键词
姜黄素/甘草次酸-PEI-PLGA纳米粒/薄膜水化法/制备工艺Key words
curcumin/glycyrrhetinic acid-PEI-PLGA nanoparticles/thin film hydration method/preparation process分类
医药卫生引用本文复制引用
胡慧中,郑晓君,胡巧红..姜黄素-甘草次酸-PEI-PLGA 纳米粒的制备及工艺优化[J].山东医药,2015,(16):8-11,4.基金项目
广东省自然科学基金资助项目（8151022401000021）。 （）
