摘要
Abstract
Objective To investigate the effects of icariin and anhydroicaritin on the proliferation and mineralization of human dental pulp cells (DPCs).Methods DPCs were divided into the observation groups A , B and the control group. Observation groups A and B were treated with icariin or anhydroicaritin at different concentrations , respectively;the control group was not treated .Cell proliferation was determined by CCK-8 assay ( OD450 represents the absorbance value at 450 nm wavelength).The real-time PCR was used to detect the mRNA expression of mineralization-related gene osteocalcin (OCN) and dentinogensis-specific gene dentin sialophosphoprotein (DSPP).Alkaline phosphatase (ALP) histochemical staining and alizarin red staining were used to evaluate the effects of icariin and anhydroicaritin on the mineralization capaci -ty of DPCs.Results After the cells were treated with 0.1, 1 and 10 μmol/L icariin, the OD450 values in the observation group A were 1.86 ±0.08, 1.96 ±0.07 and 1.82 ±0.04, respectively.After the cells were treated with 0.1, 1,10μmol/L anhydroicaritin, the OD450 values in the observation group B were 1.90 ±0.07, 1.93 ±0.09 and 1.88 ±0.02, re-spectively.Meanwhile, the OD450 value in the control group was 1.76 ±0.10.The OD450 values in the observation groups A and B were higher than that in the control group (all P<0.05).However, there was no significant difference between ob-servation groups A and B (all P>0.05).Similarly, OCN and DSPP mRNA expression in the observation groups A and B was higher than that in the control group (all P<0.05), and no significant difference was found between observation groups A and B (all P>0.05).There was no significant difference between observation groups A , B and the control group under osteoinductive condition .Conclusion Icariin and anhydroicaritin may promote proliferation and mineralization abili-ties of DPCs.However, compared with osteogenesis supplement , icariin and anhydroicaritin have a slight osteogenic effect on DPCs.关键词
淫羊藿苷/脱水淫羊藿素/牙髓细胞/增殖/成矿Key words
icariin/anhydroicaritin/dental pulp cells/proliferation/mineralization分类
医药卫生
