山东医药2015，Vol.55Issue(17)：7-9,3.DOI:10.3969/j.issn.1002-266X.2015.17.003
多色荧光原位杂交技术在尿脱落细胞学阴性膀胱癌患者术后随访监测中的应用
Application of multicolor fluorescence in situ hybridization in postoperative follow-up monitoring of bladder cancer patients with negative urine cytology
摘要
Abstract
Objective To evaluate the application value of multicolor fluorescence in situ hybridization ( M-FISH) in postoperative follow-up monitoring of bladder cancer patients with negative urine cytology .Methods A total of 78 patients with urothelial cancer who were treated by transurethral resection of the bladder were included in this study .All of them had negative urine cytology .The cystoscopy and M-FISH were used to monitor the recurrence of the disease , and the results were compared.Results In 76 patients, 14 cases recurred (18.4%).M-FISH only detected 3 cases of recurrence (4%), and 11 cases of missed diagnosis (14.5%).Cystoscopy showed 54 cases with negative results , 1 case of recur-rence (low level), and the M-FISH showed positive results.Cystoscopy showed 12 cases were suspicious and 4 cases of re-currence (2 cases of low level, 2 cases of high level), and M-FISH showed 1 case with positive results (low level).Cysto-scopy showed 10 cases with positive results , 9 cases of recurrence ( 5 cases of low level , 4 cases of high level ) , and M-FISH showed 1 case with positive results ( high level ) .For that cystoscopy showed negative results , M-FISH showed posi-tive results, the sensitivity of predicting recurrence was 100%, the specificity was 94.3%, the positive predictive value was 25%, and the negative predictive value was 100%.For that cystoscopy showed suspicious results , M-FISH showed positive results, the sensitivity of predicting recurrence was 25%, the specificity was 87.5%, the positive predictive value was 50%, and the negative predictive value was 70%.For that cystoscopy showed positive results , M-FISH showed posi-tive results, the sensitivity of predicting recurrence was 11.1%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 11.1%.Conclusions M-FISH assay in postoperative follow-up monito-ring of bladder cancer patients with negative urine cytology had a low detection rate and low economic benefits .It was not considered as a follow-up routine monitoring project in bladder cancer surveillance after transurethral resection .关键词
膀胱肿瘤/多色荧光原位杂交技术/尿脱落细胞学Key words
urinary bladder neoplasms/multicolor fluorescence in situ hybridization/urine cytology分类
医药卫生引用本文复制引用
王南雄,王尉,吕军,王健,叶纯..多色荧光原位杂交技术在尿脱落细胞学阴性膀胱癌患者术后随访监测中的应用[J].山东医药,2015,55(17):7-9,3.基金项目
国家自然科学基金项目(81372744). （81372744）
