山东医药Issue(18)：14-17,4.DOI:10.3969/j.issn.1002-266X.2015.18.005
阻断 RAAS 不同环节对肝纤维化大鼠 ACE2、AngⅡ、Ang 1-7的影响
Effects of local blockage of RAAS at different levels on expression of ACE2, AngⅡand Ang 1-7 in rats with hepatic fibrosis
摘要
Abstract
Objective To observe the effects of local blockage of renin-angiotensin-aldosterone system ( RAAS) on the degrees of fibrosis and to investigate its mechanism in liver tissues of experimental rats with hepatic fibrosis .Methods Fifty SD rats aged 6 weeks were randomly divided into five groups: the normal control group , model group , captopril group, losartan group and spironolactone group , with 10 rats in each group .In the normal control group , the rats received subcutaneous injection of olive oil , while other rats received subcutaneous injection of 40%CCL4 to make the hepatic fibro-sis models.The next day, rats of the three treatment groups were daily injected with captopril (captopril group), losartan ( losartan group ) and spironolactone ( spironolactone group ) .Rats in the control group and model group received the same amount of normal saline everyday .Rats in each group were sacrificed at 8 weeks.The liver tissues were obtained and stained by HE staining and Masson staining to observe the pathological changes .Enzyme-linked immunosorbent assay ( ELISA) was used to determine the levels of ACE 2, AngⅡand Ang 1-7 in the serum and liver tissues .Results The de-gree of liver fibrosis in the captopril group , losartan group and spironolactone group was significantly improved as compared with that of the model group;the levels of ACE2, AngⅡ, Ang 1-7 in the serum and liver tissue of the model group , capto-pril group, losartan group and spironolactone group were all higher than those of the normal control group ; the levels of ACE2 and Ang 1-7 in the captopril group , losartan group and spironolactone group were all higher , but the Ang Ⅱ level was lower than that of the model group (all P<0.05).No statistically significant differences were found in the levels of ACE2, Ang Ⅱ, and Ang 1-7 between the losartan group , spironolactone group and captopril group .Conclusion The lo-cal blockage of RAAS at different levels may all inhibit the liver fibrosis , whose mechanism may be related with the increase of ACE2 and Ang 1-7 and the decrease of AngⅡin the serum and liver tissues .关键词
肾素-血管紧张素-醛固酮系统/肝纤维化/血管紧张素Ⅱ/血管紧张素1-7Key words
renin-angiotensin-aldosterone system/liver fibrosis/angiotensin Ⅱ/angiotensin 1-7分类
医药卫生引用本文复制引用
张晓梅,焦占江,殷鹏,丁国善,周杰,崔忠林,钱建平,曾德华..阻断 RAAS 不同环节对肝纤维化大鼠 ACE2、AngⅡ、Ang 1-7的影响[J].山东医药,2015,(18):14-17,4.基金项目
广东省自然科学基金资助项目（S2011010003901）。 （）
