陕西中医Issue(7)：925-928,4.DOI:10.3969/j.issn.1000-7369.2015.07.082
红景天苷对百草枯诱发PC12细胞损伤的保护作用
The protective effects of salidroside against paraquat-induced PC12 Cell line damage
摘要
Abstract
Objective:To explore the neuroprotective effect and related mechanisms of Salidroside against pa‐raquat(PQ) induced the damage in pheochromocytoma (PC12) cells .Methods :Using PQ‐induced PC12 cells damage as the model of Parkinson’ s disease in vitro ,Cells were divided into 3 groups:control group;PQ treated group:800 μmol/L PQ treated alone;preventive groups :(10 ,20 ,30)μmol/L salidroside + 800 μmol/L PQ .the cytotoxicity of PQ was measured by MTT assay ,the apoptosis of PC12 cells was detected by flow cytometry ,Mitochondrial membrane potential (MMP)of PC12 cells was detected by Rh123 staining ,the release of LDH and the active of SOD ,GSH‐PX were detected by shade selection assay ,CytC immunostaining were used to detect the expresion of this protein .Results :10 ,20 ,30μmol/L Salidroside played a protective role in the damage induced by 800 μmol/L PQ for 24 h .Salidroside significantly increased the cell viability ,inhibited LDH ,CytC release induced by PQ .MMP collaps in preventive groups were inhibited and the active of SOD ,GSH‐PX were markedly enhanced .Conclusion:Salidroside can protect cells from the necrosis and apoptosis induced by PQ ,The mechanism of the protective effects of Salidroside may include increasing the activation of active of SOD ,GSH‐PX ,inhibiting the release of CytC and MMP collapse induced by PQ .关键词
红景天苷/百草枯/细胞凋亡/PC12细胞/神经保护Key words
Salidroside/PC12 cells Paraquat/Cenapoptosis/Neuroprotective分类
医药卫生引用本文复制引用
李海龙,吴守振,李洺,冯晓霞..红景天苷对百草枯诱发PC12细胞损伤的保护作用[J].陕西中医,2015,(7):925-928,4.基金项目
国家自然科学基金项目 （）
