上海农业学报Issue(3)：20-25,6.
进境棉籽中棉花枯萎病菌的检疫鉴定和监控策略
Identification and monitoring strategy of Fusarium oxysporum f.sp. vasinfectum from imported cotton seeds
摘要
Abstract
FOV-093,FOV-130 and FOV-245 were isolated from imported cotton seeds of Australia by using traditional PDA isolation method.These isolates produced typical macroconidia and microconidia of Fusarium oxysporum f.sp.vasinfectum .Macroconidia were falciform,oval;microconidia gathered to head shape,single cell,spawn.The general primer ITS4/ITS5 and the specific primer FOV-F/R yielded PCR products of approximately 518 bp and 354 bp,respectively.Compared with the identity of rDNA sequence of Fusarium oxysporum f.sp.vasinfectum from NCBI GenBank databases by BLAST method,the results showed that its homology was up to 100%.Pathogenicity tests showed that cotton seedlings were infected,wilted to death by FOV-093,FOV-130 and FOV-245. Based on morphological characteristics,molecular and pathogenicity test results,FOV-093,FOV-130 and FOV-245 were identified as Fusarium oxysporum f.sp.vasinfectum (Atk.)Snyder &Hansen.It was an effective way to prevent this fungus from proliferating and spreading by strengthening cotton seeds pest monitoring,inspection and quarantine.关键词
进境棉籽/棉花枯萎病菌/分离/鉴定/监控策略Key words
Imported cotton seeds/Fusarium oxysporum f.sp. vasinfectum/Isolation/Identification/Monitoring strategy分类
农业科技引用本文复制引用
孙娟,陈舜胜,乔艳艳,于子翔,于翠,杨翠云..进境棉籽中棉花枯萎病菌的检疫鉴定和监控策略[J].上海农业学报,2014,(3):20-25,6.基金项目
上海检验检疫局科技项目（HK010-2012）；上海市科委技术标准专项（11dz0502700）资助 （）
