上海农业学报Issue(1)：32-36,5.DOI:10.15955/j.issn1000-3924.2015.01.08
猪伪狂犬病毒C株的分离鉴定
Isolation and identification of porcine pseudorabies virus(PRV)C strain
摘要
Abstract
One porcine pseudorabies virus was isolated from epidemiological investigation,which was named PRV C strain,then the specificity of the strain was studied.The special prime during the region gI and gE was designed to check the strain by PCR,it showed that objective band of Min A strain lied at about 5 000 bp,but the strain lied at about 1 500 bp gene sequencing showed that the sequencing was 1 538 bp.The gene deletion of the strain was during 989—4 468,about 3 570 bp.The deletion consisted of the main virulence(gE),the muta-tions happened at the site of 873,4 665,4 666,4 950.The homology of the strain and Bartha-K61 was during 95.0%—96.0%,so it was different from the Bartha-K61.The neutralization test was done on neutralization of the virus antibody by the 14 strains PRV isolated from some provinces in China and Min A strain.The neutraliza-tion indexes were 708—6 761,the strain could neutralize all the strains PRV,which indicated that it could be the candidate vaccine strain.关键词
猪伪狂犬病病毒/C株/中和试验/分离鉴定Key words
Porcine pseudorabies virus(PRV)/C Strain/Neutralization test/Isolation and identification引用本文复制引用
高俊锋,赖志,舒银辉,漆世华,马晶晶,吴碧清,龚建培..猪伪狂犬病毒C株的分离鉴定[J].上海农业学报,2015,(1):32-36,5.基金项目
上海市中小型企业科学技术创新扶持资金项目 （）
