上海农业学报Issue(2)：18-21,4.DOI:10.15955/j.issn1000-3924.2015.02.04
克隆猪隐睾症雌雄激素及其受体基因表达的研究
Expression of testosterone,estrogen and their receptors in cryptorchidism of cloned pigs
摘要
Abstract
In this study,the serum levels of testosterone(T)and estrogen(E2 )were detected by fluorescent immunocytometry technology in 3 cloned pigs with cryptorchidism and 3 normal pigs at different stages(2—6 months old).RT-PCR technique was used to analysis the mRNA expressions of AR and ER gene in testicular tis-sue.The results showed that the serum levels of T and E2 in normal pigs increased with their growth and develop-ment,and the trend of E2 in the cloned pigs was similar with those of normal pigs.The concentration of T in-creased and reached the highest in 5 months old[(1.98 ±1.06)ng?mL],then decreased.Serum levels of Tin cloned pigs were much lower than those of normal pigs.The expression levels of ER gene in cloned pigs were higher than that of normal pigs,but there were no significant differences between them.On the contrary,the ex-pression levels of AR gene in cloned pigs were much lower than those of normal pigs.Results indicated that the lower serum level of T and the abnormal expression of AR gene may be the important factors for the occurrence of cryptorchidism in cloned pigs.关键词
克隆猪/隐睾/睾酮/雌激素/受体/基因表达Key words
Cloned pig/Cryptorchidism/Testosterone/Estrogen/Receptor/Gene expression引用本文复制引用
李芳芳,戴建军,吴彩凤,张树山,王昕,吴軻龙..克隆猪隐睾症雌雄激素及其受体基因表达的研究[J].上海农业学报,2015,(2):18-21,4.基金项目
上海市科委农业成果转化专项（123919N0700，133919N1700）；国家自然科学基金（31372315）；国家转基因生物新品种培育科技重大专项 （）
