上海针灸杂志Issue(1)：63-66,4.DOI:10.13460/j.issn.1005-0957.2015.01.0063
“双固一通”电针法对老年阳虚证模型大鼠肝线粒体结构及ATP酶活性的影响
Effect of “Double Reinforcing and One Unblocking” Electroacupuncture on Hepatic Mitochondrial Structure and ATPase Activity in a Rat Model of Senile Yang Deficiency
摘要
Abstract
Objective To explore the effect of “double reinforcing and one unblocking” electroacupuncture on hepatic mitochondrial structure and ATPase activity in a rat model of senile yang deficiency. Methods Forty 5-month-old male SD rats were selected and randomly allocated to groups A, B, C and D, 10 rats each. Group A was a normal control group. A model of senile yang deficiency was made by 40 days’ subdermal injection of D-galactose and then 7 days’ intramuscular injection of hydrocortisone in the other three groups of rats. Group C received treatment with “double reinforcing and one unblocking”electroacupuncture at points Guangyuan(CV4), Zusanli(ST36) and Baihui(GV20); group D, treatment with electroacupuncture at points Zhongji(CV3), Yinlingquan(SP9) and Yintang(GV29). Treatment was given 6 times a week for a total of 4 weeks. After treatment, the anti-fatigue ability was determined by swimming exhaustion time, hepatic mitochondrial structure was observed under a transmission electron microscope and hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities were measured by colorimetry in every group of rats. Results The mean swimming exhaustion time shortened significantly (all P<0.01); hepatic mitochondrial inner and outer membranes were blurred, mitochondrial ridges deformed, ruptured or disappeared and mitochondria swelled; both Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities decreased (P<0.01, P<0.05) in groups B, C and D of rats compared with group A. Swimming exhaustion time lengthened significantly (P<0.01), hepatic mitochondrial structure improved significantly and both Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities increased significantly (P<0.01, P<0.05) in group C compared with group B. Rat mean swimming exhaustion time shortened (P<0.05), hepatic rough endoplasmic reticula degranulated and hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities decreased (P<0.05) in group D compared with group B. Conclusion “Double reinforcing and one unblocking”electroacupuncture can improve anti-fatigue ability, ameliorate hepatic mitochondrial ultrastructure, increase hepatic Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities and promote hepatic energy metabolism in a rat model of senile yang deficiency.关键词
针刺疗法/电针/阳虚证/肝线粒体/ATP酶/“双固一通”电针法/大鼠Key words
Acupuncture therapy/Electroacupuncture/Yang deficiency syndrome/Hepatic mitochondria/ATPase/“Double reinforcing and one unblocking”electroacupuncture/Rats分类
医药卫生引用本文复制引用
刘建民,吕景芳,王华,刘静,陈明扬,王静芝..“双固一通”电针法对老年阳虚证模型大鼠肝线粒体结构及ATP酶活性的影响[J].上海针灸杂志,2015,(1):63-66,4.基金项目
湖北省自然科学基金重点项目(2008CDA051) （2008CDA051）
