世界中医药Issue(6)：774-777,780,5.DOI:10.3969/j.issn.1673-7202.2014.06.027
辛开苦降方对初发2型糖尿病KKA y小鼠胰岛素敏感性及糖脂代谢的影响
Effects of Xinkaikujiang Formula on Insulin Sensitivity and Glucolipid Metabolism in KKAy Mice with Incipient Type 2 Diabetes Mellitus
摘要
Abstract
Objective:To observe the intervention effects of XinkaiKujiang formula on insulin sensitivity and glucolipid metabolism in KKAy mice with incipient type 2 diabetes mellitus (T2DM).Methods:KKAy mice with type 2 diabetes mellitus were randomly assigned into the following four groups according to blood glucose levels:the model group,Rosiglitazone group,XinKaiKuJiang-low dose group (XK-L),XinKaiKuJiang-high dose(XK-H)group.Ten male C57BL/6J mice (10 weeks old)served as a non-diabetic control group. The mice in the control and model groups were intragastrically administered with 0.5% sodium carboxymethyl cellulose solution.Blood and tissue samples were collected after 4 weeks of treatment.Contents of fasting plasma glucose (FPG),fasting insulin (FINS),blood lipid,hepatic glycogen and liver lipid were detected,and pathological morphology in liver was observed under the microscope.Results:After 4 weeks of treatment,compared with the normal group,the insulin sensitivity index (ISI)in the model group was apparently de-creased (P<0.01).The levels of FPG,FINS,total cholesterol (TC),triglyceride (TG),free fatty acid (FFA),liver total cholesterol and liver triglycerides were significantly increased (P<0.01).The content of hepatic glycogen was decreased (P<0.01).Compared with the model group,ISI in the Rosiglitazone group was improved(P<0.01).FPG,FINS,TC,TG,FFA,and liver total cholesterol were significantly decreased (P<0.01,P<0.05).The content of hepatic glycogen was significantly increased (P<0.01).FINS,ISI,con-tent of hepatic glycogen in the XK-L group and XK-H group were all higher than those in the model group (P<0.05,P<0.01),and the level of TG was lower than that in the model group (P<0.05,P<0.01).The levels of FPG,TC and FFA could also be decreased in XK-H group (P<0.05,P<0.01).The XK-L group also could significantly decrease the liver triglyceride level (P<0.05).According to hepatic pathology,the liver structures of mice in the normal group were normal,while the livers of mice in the model group had marked fatty degeneration.Compared with model group,there was a lighter fatty degeneration of livers in Rosiglitazone group,and fatty degenera-tion of livers was significantly improved in the XK-L group and XK-H group.Conclusion:The XinkaiKujiang formula could enhance the insulin sensitivity in KKAy mice with T2DM,reduce hepatic insulin resistance,and increase the content of hepatic glycogen,further im-prove blood sugar levels.It also could lower the lipid level in blood and liver,reduce fatty degeneration of livers,and protect the morpho-logical structure of liver tissues effectively.关键词
辛开苦降方/2型糖尿病/KKAy小鼠/胰岛素敏感性/糖脂代谢Key words
XinkaiKujiang formula/Type 2 diabetes mellitus/KKAy mice/Insulin sensitivity/Glucolipid metabolism分类
医药卫生引用本文复制引用
胡照娟,张先慧,张艳红,张冬梅,娄利霞,柳红芳..辛开苦降方对初发2型糖尿病KKA y小鼠胰岛素敏感性及糖脂代谢的影响[J].世界中医药,2014,(6):774-777,780,5.基金项目
国家自然科学基金 （）
