世界中医药Issue(10)：1364-1366,3.DOI:10.3969/j.issn.1673-7202.2014.10.033
竹节参皂苷类化合物 HPLC 指纹图谱研究
Studies on the HPLC Fingerprints of Saponins in Rhizoma Panax Japonicus
摘要
Abstract
Objective:To establish HPLC fingerprint method for quality control of saponins in Rhizoma Panax Japonicus.Methods:The HPLC method was conducted based on a Hibar C18(4.6 mm ×250 mm,5μm) column,with acetonitrile-0.1%orthophosphoric acid as mobile phase by gradient elution.The detection wavelength was at 203 nm.The flow rate was 1.0 mL・ min-1 and the column temperature was 30 ℃.Seven samples from different sources were analyzed by HPLC.Results:Twelve main marker peaks were selected in the stand-ard fingerprint and 7 peaks of saponins were identified.The similarity of 6 samples was over 0.9.Conclusion:The precision,repeatability and stability of the method were up to standard,and this method can be used for quality evaluation of Rhizoma Panax Japonicus.关键词
竹节参/皂苷类化合物/HPLC/指纹图谱Key words
Rhizoma Panax Japonicus/Saponins/HPLC/Fingerprint分类
医药卫生引用本文复制引用
邹海艳,刘珊珊,李佳,马晗,赵晖..竹节参皂苷类化合物 HPLC 指纹图谱研究[J].世界中医药,2014,(10):1364-1366,3.基金项目
国家自然科学基金（编号81403095）；北京市属高校青年拔尖人才培育项目（编号CIT＆TCD201404175，CIT＆TCD201404184）；首都中医药护理专项 （）
